Anti-Peripherin PRPH Antibody

Product Name

Anti-Peripherin PRPH Antibody

View all Peripherin Antibodies

SKU/Catalog Number

M06787-1

Size

100ul

Description

Boster Bio Anti-Peripherin PRPH Antibody catalog # M06787-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Human, Mouse, Pig, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Peripherin PRPH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06787-1)

Host

Rabbit

Contents

Supplied as an aliquot of serum plus 5mM NaN₃

Clonality

Polyclonal

Immunogen

Recombinant human peripherin expressed in and purified from E. coli

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M06787-1 is reactive to PRPH in Bovine, Human, Mouse, Pig, Rat

Applications

M06787-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

53.651kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000-1:2,000. IF/ICC and IHC: 1:2,000.

Validation Images & Assay Conditions

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Western blot analysis of tissue and cell lysates using rabbit pAb to peripherin, M06787-1, dilution 1:10,000 in green: [1] protein standard, [2] rat spinal cord, [3] mouse spinal cord, [4] pig spinal cord, [5] cow spinal cord, [6] SH-SY5Y cells and [7] PC12 cells. The major band at ~57kDa corresponds to the major peripherin protein isoform, while other bands presumably represent protein products of alternate transcripts of the peripherin gene.

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Immunofluorescent analysis of the widely used rat pheochromocytoma PC 12 cell line stained with rabbit polyclonal antibody to peripherin, M06787-1, dilution 1:2,000, in green. The blue is Hoechst staining of nuclear DNA. Peripherin, one of the Class III family of intermediate filament subunit proteins, is a major component of the PC12 cell forming a perinuclear cap, with some filaments visible in the cytoplasm.

Gene/Protein Information For PRPH (Source: Uniprot.org, NCBI)

Alternative Names

neurofilament 4 (57kD); Neurofilament 4; peripherin; PRPH1NEF4 PRPH NEF41, PRPH peripherin peripherin|neurofilament 4 (57kD)

For more info on PRPH, check out the PRPH Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRPH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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