Anti-PGC1 beta PPARGC1B Monoclonal Antibody

Product Name

Anti-PGC1 beta PPARGC1B Monoclonal Antibody

View all PGC-1 beta Antibodies

SKU/Catalog Number

M02933

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PGC1 beta PPARGC1B Monoclonal Antibody catalog # M02933. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PGC1 beta PPARGC1B Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02933)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEOI-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PGC1 beta

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02933 is reactive to PPARGC1B in Human, Mouse, Rat

Applications

M02933 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

113 kDa

Calculated molecular weight

113.222kDa

Background of PGC-1 beta

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IP 1:50

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PPARGC1B using anti-PPARGC1B antibody (M02933).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPARGC1B antigen affinity purified monoclonal antibody (Catalog # M02933) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPARGC1B at approximately 113 kDa. The expected band size for PPARGC1B is at 113 kDa.

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Western blot analysis of PGC1 beta expression in (1) HeLa cell lysate; (2) Mouse spleen lysate; (3) Rat spleen lysate.

Gene/Protein Information For PPARGC1B (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp686C1790; ERRL1; PERCFLJ14284; peroxisome proliferator-activated receptor gamma, coactivator 1 beta; PGC1; PGC1Bgamma, coactivator 1, beta; PPARGC1 PPARGC1B ERRL1, PERC, PGC-1(beta), PGC1B PPARG coactivator 1 beta peroxisome proliferator-activated receptor gamma coactivator 1-beta|PGC-1-related estrogen receptor alpha coactivator|PPAR-gamma coactivator 1-beta|PPARGC-1-beta|PPARgamma coactivator 1 beta|peroxisome proliferator-activated receptor gamma, coactivator 1 beta

For more info on PPARGC1B, check out the PPARGC1B Infographic

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In this infographic, you will see the following information for PPARGC1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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