Product Info Summary
SKU: | M01018-2 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PGP9.5 UCHL1 Rabbit Monoclonal Antibody
View all UCH-L1/PGP9.5 Antibodies
SKU/Catalog Number
M01018-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PGP9.5 UCHL1 Rabbit Monoclonal Antibody catalog # M01018-2. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PGP9.5 UCHL1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01018-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAAC-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PGP9.5
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01018-2 is reactive to UCHL1 in Human, Mouse, Rat
Applications
M01018-2 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
25 kDa
Calculated molecular weight
24.824kDa
Background of UCH-L1/PGP9.5
Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PGP95 using anti-PGP95 antibody (M01018-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGP95 antigen affinity purified monoclonal antibody (Catalog # M01018-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGP95 at approximately 25 kDa. The expected band size for PGP95 is at 25 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human prostate cancer, using PGP9.5 Antibody.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:500 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:300 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer, using the Antibody at 1:1000 dilution.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human tonsil, using the Antibody at 1:1000 dilution.
Click image to see more details
Immunofluorescent analysis of U87-MG cells, using PGP9.5 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For UCHL1 (Source: Uniprot.org, NCBI)
Gene Name
UCHL1
Full Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
Weight
24.824kDa
Superfamily
peptidase C12 family
Alternative Names
EC 3.4.19.12; EC 6.-; Neuron cytoplasmic protein 9.5; PARK5; PGP 9.5; PGP9.5; PGP9.5Uch-L1; PGP95; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin C-terminal hydrolase; Ubiquitin thioesterase L1; UCHL1; UCH-L1 UCHL1 HEL-117, HEL-S-53, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, SPG79, Uch-L1 ubiquitin C-terminal hydrolase L1 ubiquitin carboxyl-terminal hydrolase isozyme L1|epididymis luminal protein 117|epididymis secretory protein Li 53|neuron cytoplasmic protein 9.5|ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)|ubiquitin thioesterase L1|ubiquitin thiolesterase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UCHL1, check out the UCHL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UCHL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PGP9.5 UCHL1 Rabbit Monoclonal Antibody (M01018-2)
Hello CJ!
M01018-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Nude mice multi-drug resistance model of orthotopic transplantation of liver neoplasm and Tc-99m MIBI SPECT on p-glycoprotein
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PGP9.5 UCHL1 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-PGP9.5 UCHL1 Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-PGP9.5 UCHL1 Rabbit Monoclonal Antibody
Question
Will M01018-2 anti-PGP9.5 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
As indicated on the product datasheet, M01018-2 anti-PGP9.5 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-19
Question
I see that the anti-PGP9.5 Rabbit Monoclonal antibody M01018-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-13
Question
Is this M01018-2 anti-PGP9.5 Rabbit Monoclonal antibody reactive to the isotypes of UCHL1?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
The immunogen of M01018-2 anti-PGP9.5 Rabbit Monoclonal antibody is A synthesized peptide derived from human PGP9.5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-21
Question
Will anti-PGP9.5 Rabbit Monoclonal antibody M01018-2 work for WB with cajal-retzius cell fetal brain cortex?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
According to the expression profile of cajal-retzius cell fetal brain cortex, UCHL1 is highly expressed in cajal-retzius cell fetal brain cortex. So, it is likely that anti-PGP9.5 Rabbit Monoclonal antibody M01018-2 will work for WB with cajal-retzius cell fetal brain cortex.
Boster Scientific Support
Answered: 2019-08-20
Question
My colleagues were happy with the WB result of your anti-PGP9.5 Rabbit Monoclonal antibody. However we have observed positive staining in brain cytoplasm. using this antibody. Is that expected? Could you tell me where is UCHL1 supposed to be expressed?
F. Yang
Verified customer
Asked: 2019-05-30
Answer
According to literature, brain does express UCHL1. Generally UCHL1 expresses in cytoplasm. Regarding which tissues have UCHL1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 2947814
Lung, and Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-30
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cajal-retzius cell fetal brain cortex using anti-PGP9.5 Rabbit Monoclonal antibody M01018-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-30
Question
Can you help my question with product M01018-2, anti-PGP9.5 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01018-2 anti-PGP9.5 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-03
Question
Our lab want to know about to test anti-PGP9.5 Rabbit Monoclonal antibody M01018-2 on rat cajal-retzius cell fetal brain cortex for research purposes, then I may be interested in using anti-PGP9.5 Rabbit Monoclonal antibody M01018-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-02-07
Answer
The products we sell, including anti-PGP9.5 Rabbit Monoclonal antibody M01018-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-07
Question
We need using your anti-PGP9.5 Rabbit Monoclonal antibody for protein deubiquitination studies. Has this antibody been tested with western blotting on 293t cell lysate? We would like to see some validation images before ordering.
C. Bhatt
Verified customer
Asked: 2019-01-14
Answer
Thank you for your inquiry. This M01018-2 anti-PGP9.5 Rabbit Monoclonal antibody is tested on 293t cell lysate. It is guaranteed to work for IP, IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-01-14
Question
We have tried in the past anti-PGP9.5 Rabbit Monoclonal antibody for IF on anterior cingulate cortex in the past. I am using rat, and We want to use the antibody for IP next. I was wanting to use examining anterior cingulate cortex as well as brain in our next experiment. Do you have any suggestion on which antibody would work the best for IP?
C. Johnson
Verified customer
Asked: 2018-12-14
Answer
I have checked the website and datasheets of our anti-PGP9.5 Rabbit Monoclonal antibody and it appears that M01018-2 has been tested on rat in both IF and IP. Thus M01018-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-12-14
Question
See attached the WB image, lot number and protocol we used for cajal-retzius cell fetal brain cortex using anti-PGP9.5 Rabbit Monoclonal antibody M01018-2. Please let me know if you require anything else.
M. Brown
Verified customer
Asked: 2018-08-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-20
Question
Is a blocking peptide available for product anti-PGP9.5 Rabbit Monoclonal antibody (M01018-2)?
D. Jha
Verified customer
Asked: 2018-05-28
Answer
We do provide the blocking peptide for product anti-PGP9.5 Rabbit Monoclonal antibody (M01018-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-05-28
Question
I was wanting to use your anti-PGP9.5 Rabbit Monoclonal antibody for WB for rat cajal-retzius cell fetal brain cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat cajal-retzius cell fetal brain cortex identification?
Verified Customer
Verified customer
Asked: 2018-05-07
Answer
As indicated on the product datasheet, M01018-2 anti-PGP9.5 Rabbit Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cajal-retzius cell fetal brain cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-07
Question
Do you have a BSA free version of anti-PGP9.5 Rabbit Monoclonal antibody M01018-2 available?
Verified Customer
Verified customer
Asked: 2018-04-17
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PGP9.5 Rabbit Monoclonal antibody M01018-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-17
Question
We have been able to see staining in rat brain. Do you have any suggestions? Is anti-PGP9.5 Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
According to literature brain does express UCHL1. According to Uniprot.org, UCHL1 is expressed in anterior cingulate cortex, lung muscle, brain, cajal-retzius cell fetal brain cortex, among other tissues. Regarding which tissues have UCHL1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 2947814
Lung, and Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-01-29
Question
We are currently using anti-PGP9.5 Rabbit Monoclonal antibody M01018-2 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
T. Jackson
Verified customer
Asked: 2015-12-23
Answer
The anti-PGP9.5 Rabbit Monoclonal antibody (M01018-2) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-23