Product Info Summary
SKU: | A03625-2 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PHAP ANP32A Antibody
SKU/Catalog Number
A03625-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PHAP ANP32A Antibody (Catalog # A03625-2). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PHAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PHAP ANP32A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03625-2)
Host
Rabbit
Contents
PHAP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PHAP antibody was raised with a synthetic peptide corresponding to amino acids at amino terminus of human PHAP. The immunogen is located within the first 50 amino acids of PHAP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This polyclonal antibody has no cross-reaction to PHAP I2a and PHAP III.
Reactive Species
A03625-2 is reactive to ANP32A in Human, Mouse, Rat
Applications
A03625-2 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
28.585kDa
Background of ANP32A
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PHAP antibody can be used for detection of all three identified isoforms of PHAP including PHAP I, PHAP I2a, and PHAP III by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PHAP expression in human Raji cell lysate with PHAP antibody at 1 μg/mL. The wide and strong band below PHAP III is a doublelet composed of PHAP I (upper) and PHAP I2a (lower).
Click image to see more details
Immunocytochemistry of PHAP in Raji cells with PHAP antibody at 1 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ANP32A (Source: Uniprot.org, NCBI)
Gene Name
ANP32A
Full Name
Acidic leucine-rich nuclear phosphoprotein 32 family member A
Weight
28.585kDa
Superfamily
ANP32 family
Alternative Names
acidic (leucine-rich) nuclear phosphoprotein 32 family, member A; acidic leucine-rich nuclear phosphoprotein 32 family member A; Acidic nuclear phosphoprotein pp32; cerebellar leucine rich acidic nuclear protein; I1PP2A; inhibitor-1 of protein phosphatase-2A; LANPPutative HLA-DR-associated protein I; Leucine-rich acidic nuclear protein; MAPMC15orf1; mapmodulin; PHAP1MGC150373; PHAPIMGC119787; Potent heat-stable protein phosphatase 2A inhibitor I1PP2A; PP32; putative human HLA class II associated protein I ANP32A C15orf1, HPPCn, I1PP2A, LANP, MAPM, PHAP1, PHAPI, PP32 acidic nuclear phosphoprotein 32 family member A acidic leucine-rich nuclear phosphoprotein 32 family member A|acidic (leucine-rich) nuclear phosphoprotein 32 family, member A|acidic nuclear phosphoprotein pp32|cerebellar leucine rich acidic nuclear protein|epididymis secretory sperm binding protein|hepatopoietin Cn|inhibitor-1 of protein phosphatase-2A|leucine-rich acidic nuclear protein|mapmodulin|potent heat-stable protein phosphatase 2A inhibitor I1PP2A|putative HLA-DR-associated protein I|putative human HLA class II associated protein I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANP32A, check out the ANP32A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANP32A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PHAP ANP32A Antibody (A03625-2)
Hello CJ!
No publications found for A03625-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PHAP ANP32A Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-PHAP ANP32A Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question