Product Info Summary
SKU: | A07456 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PHAP III ANP32E Antibody
SKU/Catalog Number
A07456
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PHAP III ANP32E Antibody (Catalog # A07456). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PHAP III antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PHAP III ANP32E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07456)
Host
Rabbit
Contents
PHAP III Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PHAP III antibody was raised with a synthetic peptide corresponding to amino acids close to carboxy terminus of human PHAP III. The immunogen is located within amino acids 200 - 250 of PHAP III.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
PHAP III has no cross-reaction to PHAP I and PHAP I2a.
Reactive Species
A07456 is reactive to Anp32e in Human, Mouse, Rat
Applications
A07456 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
29.622kDa
Background of LANPL
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PHAP III antibody can be used for detection of PHAP III by Western blot at 1 μg/mL. A band at approximately 35 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human and rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PHAP III expression in human A549 (A) and HepG2 (B) cells
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Immunohistochemistry of PHAP III in human small intestine tissue with PHAP III antibody at 2 μg/mL.
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Immunofluorescence of PHAP III in Human Small Intestine cells with PHAP III antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Anp32e (Source: Uniprot.org, NCBI)
Gene Name
Anp32e
Full Name
Acidic leucine-rich nuclear phosphoprotein 32 family member E
Weight
29.622kDa
Superfamily
ANP32 family
Alternative Names
acidic (leucine-rich) nuclear phosphoprotein 32 family, member E; LANP-like protein; LANP-LLANPLacidic leucine-rich nuclear phosphoprotein 32 family member E; leucine-rich acidic nuclear protein like; MGC5350 Anp32e|2810018A15Rik, AI047746, AI326868, CPD, CPD1, L, LANP-L, LANPL, mLANP-L|acidic (leucine-rich) nuclear phosphoprotein 32 family, member E|acidic leucine-rich nuclear phosphoprotein 32 family member E|LANP-like protein|cerebellar postnatal development protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Anp32e, check out the Anp32e Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Anp32e: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PHAP III ANP32E Antibody (A07456)
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No publications found for A07456
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Customer Reviews
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