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Product Info Summary
| SKU: | P01420-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-AMPK alpha (T172) PRKAA2 Rabbit Monoclonal Antibody
View all AMPK alpha 2 Antibodies
SKU/Catalog Number
P01420-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-AMPK alpha (T172) PRKAA2 Rabbit Monoclonal Antibody catalog # P01420-2. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-AMPK alpha (T172) PRKAA2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01420-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EHA-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AMPK alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P01420-2 is reactive to PRKAA2 in Human, Mouse, Rat
Applications
P01420-2 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
130 kDa
Calculated molecular weight
62.32kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.
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Western blot analysis of Phospho-AMPK alpha (T172) expression in Mouse heart lysate.
Protein Target Info & Infographic
Gene/Protein Information For PRKAA2 (Source: Uniprot.org, NCBI)
Gene Name
PRKAA2
Full Name
5'-AMP-activated protein kinase catalytic subunit alpha-2
Weight
62.32kDa
Superfamily
protein kinase superfamily
Alternative Names
5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK alpha 2; AMPK subunit alpha-2; AMPK2; AMPK5'-AMP-activated protein kinase, catalytic alpha-2 chain; AMPK-alpha-2 chain; EC 2.7.11; EC 2.7.11.1; PRKAA; PRKAA2; protein kinase, AMP-activated, alpha 2 catalytic subunit PRKAA2 AMPK, AMPK2, AMPKa2, PRKAA protein kinase AMP-activated catalytic subunit alpha 2 5-AMP-activated protein kinase catalytic subunit alpha-2|5-AMP-activated protein kinase, catalytic alpha-2 chain|ACACA kinase|AMP-activated protein kinase alpha-2 subunit variant 2|AMP-activated protein kinase alpha-2 subunit variant 3|AMPK alpha 2|AMPK subunit alpha-2|AMPK-alpha-2 chain|HMGCR kinase|acetyl-CoA carboxylase kinase|hydroxymethylglutaryl-CoA reductase kinase|protein kinase, AMP-activated, alpha 2 catalytic subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKAA2, check out the PRKAA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKAA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-AMPK alpha (T172) PRKAA2 Rabbit Monoclonal Antibody (P01420-2)
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No publications found for P01420-2
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Phospho-AMPK alpha (T172) PRKAA2 Rabbit Monoclonal Antibody
Question
We are currently using anti-Phospho-AMPK alpha (T172) Rabbit Monoclonal antibody P01420-2 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-27
Answer
The anti-Phospho-AMPK alpha (T172) Rabbit Monoclonal antibody (P01420-2) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-27
