Product Info Summary
SKU: | A03884T74 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-C/EBP epsilon (T74) CEBPE Antibody
View all CEBP epsilon Antibodies
SKU/Catalog Number
A03884T74
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-C/EBP epsilon (T74) CEBPE Antibody catalog # A03884T74. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-C/EBP epsilon (T74) CEBPE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03884T74)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human C/EBP epsilon around the phosphorylation site of T74.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03884T74 is reactive to CEBPE in Human
Applications
A03884T74 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
30.603kDa
Background of CEBP epsilon
Required for transcriptional activation subsequent to the assembly of the preinitiation complex By similarity. Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.
Wiemann S., Genome Res. 11:422-435(2001).
Mungall A.J., Nature 425:805-811(2003).
Akoulitchev S., Nature 407:102-106(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Immunohistochemistry validation of CEBPE using Anti-Phospho-C/EBP epsilon (T74) CEBPE Antibody (A03884T74).
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°CProtein Target Info & Infographic
Gene/Protein Information For CEBPE (Source: Uniprot.org, NCBI)
Gene Name
CEBPE
Full Name
CCAAT/enhancer-binding protein epsilon
Weight
30.603kDa
Superfamily
bZIP family
Alternative Names
C/EBP-epsilon; CCAAT/enhancer binding protein (C/EBP), epsilon; CCAAT/enhancer-binding protein epsilon; CEBP epsilon; CEBPE; CRP1C/EBP epsilon CEBPE C/EBP-epsilon, CRP1, c/EBP epsilon CCAAT enhancer binding protein epsilon CCAAT/enhancer-binding protein epsilon|CCAAT/enhancer binding protein (C/EBP), epsilon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CEBPE, check out the CEBPE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CEBPE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-C/EBP epsilon (T74) CEBPE Antibody (A03884T74)
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No publications found for A03884T74
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Customer Reviews
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