Anti-Phospho-c-Myc (T58) Rabbit Monoclonal Antibody

c-Myc antibody

Boster Bio Anti-Phospho-c-Myc (T58) Rabbit Monoclonal Antibody catalog # P00026. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human. Cited in 5 publication(s).

Product Info Summary

SKU: P00026
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, ICC, WB

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Product Name

Anti-Phospho-c-Myc (T58) Rabbit Monoclonal Antibody

View all c-Myc Antibodies

SKU/Catalog Number

P00026

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-c-Myc (T58) Rabbit Monoclonal Antibody catalog # P00026. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-c-Myc (T58) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00026)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FOC-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Phospho-c-Myc (T58)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00026 is reactive to MYC in Human

Applications

P00026 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

65 kDa

Calculated molecular weight

48.804kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For MYC (Source: Uniprot.org, NCBI)

Gene Name

MYC

Full Name

Myc proto-oncogene protein

Weight

48.804kDa

Alternative Names

9E10 Myc; 9E10 Myc; avian myelocytomatosis viral oncogene homolog; BHLHE39; bHLHe39MRTL; Class E basic helix-loop-helix protein 39; cMyc; c-Myc; myc proto-oncogene protein; Myc; Myc2; MYCC; myc-related translation/localization regulatory factor; Niard; Nird; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian) MYC MRTLC, bHLHe39, c-Myc, MYC MYC proto-oncogene, bHLH transcription factor myc proto-oncogene protein|avian myelocytomatosis viral oncogene homolog|class E basic helix-loop-helix protein 39|myc-related translation/localization regulatory factor|proto-oncogene c-Myc|transcription factor p64|v-myc avian myelocytomatosis viral oncogene homolog|v-myc myelocytomatosis viral oncogene homolog

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MYC, check out the MYC Infographic

MYC infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MYC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

P00026 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Inhibition of N-myc downstream-regulated gene 2 in prostatic carcinoma

Tumor suppressor NDRG2 inhibits glycolysis and glutaminolysis in colorectal cancer cells by repressing c-Myc expression

Blockage of Glyoxalase I Inhibits Colorectal Tumorigenesis and Tumor Growth via Upregulation of STAT1, p53, and Bax and Downregulation of c-Myc and Bcl-2

Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H, Liu M. Plos One. 2013 Oct 23;8(10):E77829. Doi: 10.1371/Journal.Pone.0077829. Ecollection 2013. Down-Regulation Of Mir-129-5P Inhibits Growth And Induces Apoptosis In Laryngeal Squa...

Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.

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5 Customer Q&As for Anti-Phospho-c-Myc (T58) Rabbit Monoclonal Antibody

Question

Our lab were satisfied with the WB result of your anti-Phospho-c-Myc (T58) Rabbit Monoclonal antibody. However we have seen positive staining in cervix carcinoma nucleoplasm using this antibody. Is that expected? Could you tell me where is MYC supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-02-21

Answer

According to literature, cervix carcinoma does express MYC. Generally MYC expresses in nucleus, nucleoplasm. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-02-21

Question

We have tried in the past anti-Phospho-c-Myc (T58) Rabbit Monoclonal antibody for WB on placenta testis a few months ago. I am using human, and We intend to use the antibody for IF next. My lab would like examining placenta testis as well as cervix carcinoma erythroleukemia in our next experiment. Do you have any suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2019-11-25

Answer

I have checked the website and datasheets of our anti-Phospho-c-Myc (T58) Rabbit Monoclonal antibody and I see that P00026 has been validated on human in both WB and IF. Thus P00026 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-11-25

Question

I was wanting to use using your anti-Phospho-c-Myc (T58) Rabbit Monoclonal antibody for positive regulation of telomerase activity studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-02-08

Answer

Thank you for your inquiry. This P00026 anti-Phospho-c-Myc (T58) Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-02-08

Question

We have observed staining in human thoracic mammary gland. Are there any suggestions? Is anti-Phospho-c-Myc (T58) Rabbit Monoclonal antibody supposed to stain thoracic mammary gland positively?

B. Jones

Verified customer

Asked: 2016-09-21

Answer

From literature thoracic mammary gland does express MYC. From Uniprot.org, MYC is expressed in thoracic mammary gland, uterus, cervix, placenta testis, promyelocytic leukemia, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2016-09-21

Question

We are currently using anti-Phospho-c-Myc (T58) Rabbit Monoclonal antibody P00026 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?

D. Zhao

Verified customer

Asked: 2015-11-10

Answer

The anti-Phospho-c-Myc (T58) Rabbit Monoclonal antibody (P00026) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-11-10

Order DetailsPrice
P00026

100μL

$370
P00026-30ul

30μL sample

$99
P00026-carrier-free

Carrier Free

$370

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