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Product Info Summary
| SKU: | P01060 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-Chk1 (S296) CHEK1 Rabbit Monoclonal Antibody
SKU/Catalog Number
P01060
BM4516 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Chk1 (S296) CHEK1 Rabbit Monoclonal Antibody catalog # P01060. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Chk1 (S296) CHEK1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01060)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FEO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Chk1 (S296)
Reactive Species
P01060 is reactive to CHEK1 in Human
Observed Molecular Weight
54 kDa
Calculated molecular weight
54.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01060 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: HEK293 cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-Chk1 (S296) expression in HEK293 cell lysate Treated with Calyculin.
Specific Publications For Anti-Phospho-Chk1 (S296) CHEK1 Rabbit Monoclonal Antibody (P01060)
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Customer Q&As
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3 Customer Q&As for Anti-Phospho-Chk1 (S296) CHEK1 Rabbit Monoclonal Antibody
Question
We have observed staining in human brain testis. What should we do? Is anti-Phospho-Chk1 (S296) Rabbit Monoclonal antibody supposed to stain brain testis positively?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
From literature brain testis does express CHEK1. From Uniprot.org, CHEK1 is expressed in secondary oocyte, fetal thymus, brain testis, bone marrow muscle, embryonic kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CHEK1 expression, here are a few articles citing expression in various tissues:
Bone marrow, and Muscle, Pubmed ID: 15489334
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal thymus, Pubmed ID: 22184239
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2020-04-30
Question
We are currently using anti-Phospho-Chk1 (S296) Rabbit Monoclonal antibody P01060 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
The anti-Phospho-Chk1 (S296) Rabbit Monoclonal antibody (P01060) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-30
Question
My boss were content with the WB result of your anti-Phospho-Chk1 (S296) Rabbit Monoclonal antibody. However we have observed positive staining in leukemic t-cell nucleus. cytoplasm. cytoplasm using this antibody. Is that expected? Could you tell me where is CHEK1 supposed to be expressed?
G. Rodriguez
Verified customer
Asked: 2013-10-10
Answer
From literature, leukemic t-cell does express CHEK1. Generally CHEK1 expresses in nucleus. cytoplasm. cytoplasm, cytoskeleton,. Regarding which tissues have CHEK1 expression, here are a few articles citing expression in various tissues:
Bone marrow, and Muscle, Pubmed ID: 15489334
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fetal thymus, Pubmed ID: 22184239
Leukemic T-cell, Pubmed ID: 19690332
Boster Scientific Support
Answered: 2013-10-10
