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Product Info Summary
| SKU: | P00543 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-Cyclin E1 (T77) CCNE1 Rabbit Monoclonal Antibody
SKU/Catalog Number
P00543
BM4653 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Cyclin E1 (T77) CCNE1 Rabbit Monoclonal Antibody catalog # P00543. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Cyclin E1 (T77) CCNE1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00543)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GHC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cyclin E1
Reactive Species
P00543 is reactive to CCNE1 in Human
Observed Molecular Weight
48 kDa
Calculated molecular weight
47.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00543 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: JAR cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-Cyclin E1 (T77) expression in (1) JAR cell treated with Lambda Phosphatase lysate; (2) JAR cell lysate.
Specific Publications For Anti-Phospho-Cyclin E1 (T77) CCNE1 Rabbit Monoclonal Antibody (P00543)
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1 Customer Q&As for Anti-Phospho-Cyclin E1 (T77) CCNE1 Rabbit Monoclonal Antibody
Question
We are currently using anti-Phospho-Cyclin E1 (T77) Rabbit Monoclonal antibody P00543 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
The anti-Phospho-Cyclin E1 (T77) Rabbit Monoclonal antibody (P00543) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-18
