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Product Info Summary
| SKU: | P00104-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-ERK1 (T202) + ERK2 (T185) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00104-2
BM5446 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Phospho-ERK1 (T202) + ERK2 (T185) Rabbit Monoclonal Antibody catalog # P00104-2. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-ERK1 (T202) + ERK2 (T185) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00104-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
18M06
Isotype
IgG
Immunogen
A synthesized peptide derived from human Phospho-ERK1 (T202) + ERK2 (T185)
Reactive Species
P00104-2 is reactive to MAPK3 in Human, Mouse, Rat
Observed Molecular Weight
42, 44 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00104-2 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:40
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of Phospho-ERK1 (T202) + ERK2 (T185) expression in (1) NIH/3T3 cell lysate; (2) NIH/3T3 cell lysate treated with PDGF.
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The effect of E343 mutation on MAPK signaling. The stably overexpressing HeLa cells were serum-starved and then exposed to SDF-1 (50 nM) and over a range of time periods (5 min to 60 min). Western blotting was performed for phosphorylated ERK1/2 ( a ), p38 ( c ) and JNK1/2/3 ( e ). Total ERK1/2, p38 and JNK were used as loading control. The relative phosphorylation level was quantified using ImageJ software and showed in ( b ) for ERK1/2, ( d ) for p38 and ( f ) for JNK1/2/3, respectively. Bars represent the mean value ± standard deviation of at least three independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001
Index in PubMed under a CC BY license. PMID: 33485325
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
Specific Publications For Anti-Phospho-ERK1 (T202) + ERK2 (T185) Rabbit Monoclonal Antibody (P00104-2)
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