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Product Info Summary
| SKU: | P01172 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-GCN2 (T899) EIF2AK4 Rabbit Monoclonal Antibody
SKU/Catalog Number
P01172
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-GCN2 (T899) EIF2AK4 Rabbit Monoclonal Antibody catalog # P01172. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-GCN2 (T899) EIF2AK4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01172)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABH-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-GCN2 (T899)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P01172 is reactive to EIF2AK4 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
186911 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01172 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: HeLa cell, HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of Phospho-GCN2 (Thr899) in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Calyculin.
Protein Target Info & Infographic
Gene/Protein Information For EIF2AK4 (Source: Uniprot.org, NCBI)
Gene Name
EIF2AK4
Full Name
eIF-2-alpha kinase GCN2
Weight
186911 MW
Superfamily
protein kinase superfamily
Alternative Names
Eukaryotic translation initiation factor 2-alpha kinase 4;2.7.11.1;GCN2-like protein;EIF2AK4;GCN2, KIAA1338; Eif2ak4|2610011M03, GCN, GCN2, MGCN2|eukaryotic translation initiation factor 2 alpha kinase 4|eIF-2-alpha kinase GCN2|GCN2 eIF2alpha kinase|GCN2-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF2AK4, check out the EIF2AK4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF2AK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-GCN2 (T899) EIF2AK4 Rabbit Monoclonal Antibody (P01172)
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No publications found for P01172
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-GCN2 (T899) EIF2AK4 Rabbit Monoclonal Antibody
Question
We are currently using anti-Phospho-GCN2 (T899) Rabbit Monoclonal antibody P01172 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
The anti-Phospho-GCN2 (T899) Rabbit Monoclonal antibody (P01172) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-02
Question
We need using your anti-Phospho-GCN2 (T899) Rabbit Monoclonal antibody for cellular response to leucine starvation studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
R. Gonzalez
Verified customer
Asked: 2019-04-29
Answer
We appreciate your inquiry. This P01172 anti-Phospho-GCN2 (T899) Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-04-29
Question
Our team were content with the WB result of your anti-Phospho-GCN2 (T899) Rabbit Monoclonal antibody. However we have observed positive staining in cervix carcinoma cytoplasm. using this antibody. Is that expected? Could you tell me where is EIF2AK4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-03-29
Answer
Based on literature, cervix carcinoma does express EIF2AK4. Generally EIF2AK4 expresses in cytoplasm. Regarding which tissues have EIF2AK4 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10718198
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Melanoma, and Testis, Pubmed ID: 17974005
Muscle, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-03-29
Question
We have seen staining in human adenohypophysis. Any tips? Is anti-Phospho-GCN2 (T899) Rabbit Monoclonal antibody supposed to stain adenohypophysis positively?
Verified Customer
Verified customer
Asked: 2018-06-11
Answer
According to literature adenohypophysis does express EIF2AK4. According to Uniprot.org, EIF2AK4 is expressed in adenohypophysis, muscle uterus, brain, melanoma testis, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have EIF2AK4 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10718198
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Melanoma, and Testis, Pubmed ID: 17974005
Muscle, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-06-11
