Product Info Summary
| SKU: | A00070Y1161-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-IGF-IR (Y1161) IGF1R Antibody
SKU/Catalog Number
A00070Y1161-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-IGF-IR (Y1161) IGF1R Antibody catalog # A00070Y1161-1. Tested in WB, IHC, IF, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-IGF-IR (Y1161) IGF1R Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00070Y1161-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human IGF1R around the phosphorylation site of Tyr1161. AA range:1131-1180
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00070Y1161-1 is reactive to IGF1R in Human, Mouse, Rat
Calculated molecular weight
154.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00070Y1161-1 is guaranteed for ELISA, IP, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IP 2-5 ug/mg lysate
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Phospho-IGF-IR (Y1161) Polyclonal Antibody diluted at 1:2000
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Western Blot analysis of 293 cells using Phospho-IGF-IR (Y1161) Polyclonal Antibody diluted at 1:2000
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Western blot analysis of lysates from 293 cells treated with Insulin, using IGF1R (Phospho-Tyr1161) Antibody. The lane on the left is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IGF1R (Phospho-Tyr1161) Antibody. The picture on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho-IGF-IR (Y1161) IGF1R Antibody (A00070Y1161-1)
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1 Customer Q&As for Anti-Phospho-IGF-IR (Y1161) IGF1R Antibody
Question
I want to purchase the Anti-Phospho-IGF-IR (Y1161) IGF1R Antibody, but I would like to know if this antibody does not cross react with phosphorylated insulin receptor.
Verified Customer
Verified customer
Asked: 2018-02-12
Answer
The product Anti-Phospho-IGF-IR (Y1161) IGF1R Antibody have been shown to cross react with insulin receptor at phosphorylation site Y1185.
Boster Scientific Support
Answered: 2018-02-12

