Product Info Summary
SKU: | P30449 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Phospho IL-7R/CD127 (Tyr449) Antibody
View all IL-7R alpha/CD127 Antibodies
SKU/Catalog Number
P30449
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho IL-7R/CD127 (Tyr449) Antibody (Catalog# P30449). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho IL-7R/CD127 (Tyr449) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30449)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
BEF-20
Isotype
Rabbit IgG
Immunogen
A peptide derived from human IL-7R/CD127 around the phosphorylation site of Tyr449. Immunogen sequence location: 410-459
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P30449 is reactive to IL7R in Human, Mouse
Applications
P30449 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
51.579kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from COLO205 cells, using IL-7R/CD127 (Phospho-Tyr449) Antibody. The lane on the right is blocked with the phospho peptide.
Click image to see more details
Immunofluorescence analysis of HUVEC cells, using IL-7R/CD127 (Phospho-Tyr449) Antibody. The picture on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For IL7R (Source: Uniprot.org, NCBI)
Gene Name
IL7R
Full Name
Interleukin-7 receptor subunit alpha
Weight
51.579kDa
Superfamily
type I cytokine receptor family
Alternative Names
CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 R alpha; IL-7 receptor subunit alpha; IL7R alpha; IL-7R alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7Ra; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha IL7R CD127, CDW127, IL-7R-alphaA, ILRA, IL7R interleukin 7 receptor interleukin-7 receptor subunit alpha|CD127 antigen|IL-7 receptor subunit alpha|IL-7R subunit alpha|interleukin 7 receptor alpha chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL7R, check out the IL7R Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL7R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho IL-7R/CD127 (Tyr449) Antibody (P30449)
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No publications found for P30449
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