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- Table of Contents
Mlkl antibody (P00535) performs well in IP, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
Filter:
Western blot analysis of Phospho-MLKL (S345) expression in (1) L929 treated with Z-VAD + TNF alpha + SM164 cell lysate; (2) Untreated.
Designing a Western blot for MLKL? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the MLKL Western Blot GuideBundle Save
Anti-Phospho-MLKL (S345) Rabbit Monoclonal Antibody
Mlkl antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | IP, WB |
Tested Applications
Mlkl antibody was positively detected by WB in:
L929
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IP 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Phospho-MLKL (S345) Rabbit Monoclonal Antibody
View all MLKL Antibodies
View all MLKL Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
27M64
Isotype
IgG
Immunogen
A synthesized peptide derived from human Phospho-MLKL (S345)
Observed Molecular Weight
54 kDa
Calculated Molecular Weight
54.3 kDa
Cite This Product
Anti-Phospho-MLKL (S345) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00535)
Protocols
Western Blot protocol for Mlkl antibody
Publications
Application:
Species:
The role and mechanism of TNFRSF21 in promoting necroptosis of vascular endothelial cells and inducing vascular leakage in sepsis
Published: 2026-04-23
Journal: JOURNAL OF MOLECULAR MEDICINE-JMM
Application: WB
Impact Factor: 4.20
Species: Rat
PMID: 42020785
FAK/SRC-JNK axis promotes ferroptosis via upregulating ACSL4 expression
Published: 2026-03-20
Journal: Cell Death & Disease
Application: WB
Impact Factor: 9.60
Species: Mouse
PMID: 41862445
IER3 Promotes Non-Small Cell Lung Cancer Malignancy by Suppressing Ferroptosis via the AKT/GSK3β/NRF2 Pathway
Published: 2026-01-23
Journal: FREE RADICAL BIOLOGY AND MEDICINE
Application: WB
Impact Factor: 8.20
Species: Human
PMID: 41581578
NIR-responsive CN-Pt-GEM hydrogel induces necroptosis and immunotherapeutic responses prevent postoperative recurrence and wound infection in lung carcinoma
Published: 2024-06-21
Journal: JOURNAL OF NANOBIOTECHNOLOGY
Application: WB
Impact Factor: 10.60
Species: Mouse,Human
PMID: 38902678
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Product Promise
We promise Anti-Phospho-MLKL (S345) Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
P00535
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 23, 2026