Anti-Phospho-NMDA Receptor NR2B Subunit (Ser1480) Grin2b Antibody

Product Name

Anti-Phospho-NMDA Receptor NR2B Subunit (Ser1480) Grin2b Antibody

View all GRIN2B Antibodies

SKU/Catalog Number

P01883-4

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-NMDA Receptor NR2B Subunit (Ser1480) Grin2b Antibody (Catalog # P01883-4). Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-NMDA Receptor NR2B Subunit (Ser1480) Grin2b Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01883-4)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Clone Number

608

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1480 of the NR2B subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P01883-4 is reactive to GRIN2B in Mouse, Rat

Applications

P01883-4 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

166.367kDa

Background of GRIN2B

The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). It has been shown that phosphorylation of Ser-1480 disrupts the interaction of NR2B with the PDZ domains of PSD-95 and SAP102 and decreases surface NR2B expression in neurons (Chung et al., 2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 min).

Gene/Protein Information For GRIN2B (Source: Uniprot.org, NCBI)

Alternative Names

glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor subunit epsilon-2; glutamate receptor, ionotropic, N-methyl D-aspartate 2B; hNR3; MGC142180; NMDAR2BMGC142178; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NR2B; NR3 GRIN2B DEE27, EIEE27, GluN2B, MRD6, NMDAR2B, NR2B, NR3, hNR3 glutamate ionotropic receptor NMDA type subunit 2B glutamate receptor ionotropic, NMDA 2B|N-methyl D-aspartate receptor subtype 2B|N-methyl-D-aspartate receptor subunit 3|glutamate [NMDA] receptor subunit epsilon-2|glutamate receptor subunit epsilon-2|glutamate receptor, ionotropic, N-methyl D-aspartate 2B

For more info on GRIN2B, check out the GRIN2B Infographic

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In this infographic, you will see the following information for GRIN2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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