Anti-Phospho-p27 (S178) CDKN1B Antibody

Product Name

Anti-Phospho-p27 (S178) CDKN1B Antibody

View all p27/Kip1 Antibodies

SKU/Catalog Number

A00173S178

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho-p27 (S178) CDKN1B Antibody catalog # A00173S178. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-p27 (S178) CDKN1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00173S178)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human p27 around the phosphorylation site of S178.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00173S178 is reactive to CDKN1B in Human, Mouse

Applications

A00173S178 is guaranteed for ELISA, IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

22.073kDa

Background of p27/Kip1

Anti cdk2 antibody recognizes a protein encoded by a gene member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC 1:100-1:300
ELISA 1:5000

Validation Images & Assay Conditions

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Figure 1. Immunohistochemistry validation of CDKN1B using Anti-Phospho-p27 (S178) CDKN1B Antibody (A00173S178).

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C

Gene/Protein Information For CDKN1B (Source: Uniprot.org, NCBI)

Alternative Names

CDKN1B; CDKN4; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Kip1; KIP1P27KIP1; MEN1B; MEN4; p27; p27Kip1 CDKN1B CDKN4, KIP1, MEN1B, MEN4, P27KIP1 cyclin dependent kinase inhibitor 1B cyclin-dependent kinase inhibitor 1B|cyclin-dependent kinase inhibitor 1B (p27, Kip1)

For more info on CDKN1B, check out the CDKN1B Infographic

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In this infographic, you will see the following information for CDKN1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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