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Product Info Summary
| SKU: | A00176T180Y182 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Guinea pig, Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-p38 (T180/Y182) MAPK14 Antibody
SKU/Catalog Number
A00176T180Y182
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-p38 (T180/Y182) MAPK14 Antibody catalog # A00176T180Y182. Tested in IF, WB, Flow Cytometry, IHC, ELISA applications. This antibody reacts with Human, Mouse, Rat, Guinea pig.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-p38 (T180/Y182) MAPK14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00176T180Y182)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human p38 MAPK around the phosphorylation site of Thr179 and Tyr181. AA range:151-200
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00176T180Y182 is reactive to MAPK14 in Guinea pig, Human, Mouse, Rat
Calculated molecular weight
41.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00176T180Y182 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF/ICC1:100-500
WB 1:500-2000
FC 1:50-200
IHC 1:100-500
ELISA 1:5000-20000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunofluorescence analysis of Hela cell. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p38 MAPK (Phospho-Thr179+Tyr181) Antibody
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Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Specific Publications For Anti-Phospho-p38 (T180/Y182) MAPK14 Antibody (A00176T180Y182)
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