Product Info Summary
SKU: | A01586S345 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-p47-phox (S345) NCF1 Antibody
SKU/Catalog Number
A01586S345
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-p47-phox (S345) NCF1 Antibody catalog # A01586S345. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-p47-phox (S345) NCF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01586S345)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human p47-phox around the phosphorylation site of S345.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01586S345 is reactive to Ncf1 in Human
Applications
A01586S345 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
44.667kDa
Background of Ncf1
Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (). CLDN1 acts as a coreceptor for HCV entry into hepatic cells.
Julie Dufresne and Daniel G. Cyr, Biol Reprod, May 2007; 76: 825 - 832.
Evemie Dub¨¦, Biol Reprod, Apr 2007; 10.1095/biolreprod.106.059246.
Michele A. Battle, PNAS, May 2006; 103: 8419 - 8424.
Edmund C. Chang, Endocrinology, Oct 2006; 147: 4831 - 4842.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Validation Images & Assay Conditions
Click image to see more details
Figure 3. Immunohistochemistry validation of NCF1 using Anti-Phospho-p47-phox (S345) NCF1 Antibody (A01586S345).
Immunohistochemical analysis of paraffin-embedded Human brain using Anti-Phospho-p47-phox (S345) antibody. Antibody was diluted at 1:100 (4°CClick image to see more details
Figure 1. Western blotting validation for Anti-Phospho-p47-phox (S345) NCF1 Antibody A01586S345
Western Blot (WB) analysis of Jurkat cells using Anti-Phospho-p47-phox (S345) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Click image to see more details
Figure 2. Western blotting validation for Anti-Phospho-p47-phox (S345) NCF1 Antibody A01586S345
Western Blot (WB) analysis of 3T3 lysis using Anti-Phospho-p47-phox (S345) antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For Ncf1 (Source: Uniprot.org, NCBI)
Gene Name
Ncf1
Full Name
Neutrophil cytosol factor 1
Weight
44.667kDa
Alternative Names
47 kDa neutrophil oxidase factor; FLJ79451; NADPH oxidase organizer 2; NCF-1; NCF1A; neutrophil cytosol factor 1,47 kDa autosomal chronic granulomatous disease protein; neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal1); neutrophil cytosolic factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); Neutrophil NADPH oxidase factor 1; Nox organizer 2; NOXO2NCF-47K; Nox-organizing protein 2; p47phox; SH3 and PX domain-containing protein 1A; SH3PXD1Ap47-phox Ncf1|NCF-47K, NO, NOXO2, Ncf-, Ncf-1, p47-phox, p47
For more info on Ncf1, check out the Ncf1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ncf1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-p47-phox (S345) NCF1 Antibody (A01586S345)
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