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Product Info Summary
| SKU: | P30440 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho PFKFB2 (Ser483) Antibody
SKU/Catalog Number
P30440
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho PFKFB2 (Ser483) Antibody (Catalog# P30440). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho PFKFB2 (Ser483) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30440)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human PFKFB2 around the phosphorylation site of Ser483. Immunogen sequence location: 451-500
Reactive Species
P30440 is reactive to PFKFB2 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
58.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P30440 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of Hela cells using Phospho-PFK-2 car (S483) Polyclonal Antibody diluted at 1:1000
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Western Blot analysis of various cells using Phospho-PFK-2 car (S483) Polyclonal Antibody diluted at 1:1000
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PFKFB2 (Phospho-Ser483) Antibody
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PFKFB2 (Phospho-Ser483) Antibody. The picture on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho PFKFB2 (Ser483) Antibody (P30440)
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Customer Reviews
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1 Customer Q&As for Anti-Phospho PFKFB2 (Ser483) Antibody
Question
We are currently using anti-Phospho PFKFB2 (Ser483) antibody P30440 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
D. Yang
Verified customer
Asked: 2019-09-27
Answer
The anti-Phospho PFKFB2 (Ser483) antibody (P30440) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-27
