Anti-Phospho-PKCth (Ser676) PRKCQ Antibody

Product Name

Anti-Phospho-PKCth (Ser676) PRKCQ Antibody

View all PKC theta Antibodies

SKU/Catalog Number

P01293

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-PKCth (Ser676) PRKCQ Antibody (Catalog # P01293). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-PKCth (Ser676) PRKCQ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01293)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Peptide sequence around phosphorylation site of serine 676 (R-L-S(p)-F-A) derived from Human PKCth.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P01293 is reactive to PRKCQ in Human, Mouse, Rat

Applications

P01293 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

81.865kDa

Background of PKC theta

This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.

Kristof Van Kolen, et al. (2006) FEBS J ; 273: 1843 - 1854.
Martin Villalba, et al. (2002) J. Cell Biol ; 157: 253.
Jie Zhang, et al. (2004) J. Biol. Chem ; 279: 22118 - 22123.
Castro AF, et al. (1998) Am J Physiol Cell Physiol; 275: C113 - C119.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 80kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Validation Images & Assay Conditions

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Western blot analysis of extracts from PC12 cells, treated with PMA or calf intestinal phosphatase (CIP), using PKCθ (Phospho-Ser676) antibody P01293.

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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCth(Phospho-Ser676) antibody P01293(left) or the same antibody preincubated with blocking peptide(right).

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Western blot analysis of extracts from PC12 cells untreated or treated with PMA using PKCth(Phospho-Ser676) antibody P01293.

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Immunofluorescence staining of methanol-fixed Hela cells using PKCth(Phospho-Ser676) antibody P01293.

Gene/Protein Information For PRKCQ (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.11; EC 2.7.11.13; MGC126514; MGC141919; nPKC-theta; PKC theta; PRKCQ; PRKCT; protein kinase C theta type; protein kinase C, theta PRKCQ PRKCT, nPKC-theta protein kinase C theta protein kinase C theta type

For more info on PRKCQ, check out the PRKCQ Infographic

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In this infographic, you will see the following information for PRKCQ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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