Product Info Summary
SKU: | P03629 |
---|---|
Size: | 100 µl |
Reactive Species: | Drosophila |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Phospho-Thr356 PRAS40 Antibody, Phospho-Specific
SKU/Catalog Number
P03629
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Thr356 PRAS40 Antibody, Phospho-Specific (Catalog # P03629). Tested in WB applications. This antibody reacts with Drosophila.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-Thr356 PRAS40 Antibody, Phospho-Specific (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03629)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr356 of Drosophila PRAS40, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Drosophila.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P03629 is reactive to AKT1S1 in Drosophila
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
27383 MW
Background of PRAS40
PRAS40 (proline-rich Akt/PKB substrate of 40 kDa) acts at the intersection of the Akt- and mTOR-mediated signaling pathways and its phosphorylation by Akt and by mTORC1 results in dissociation of PRAS40 from mTORC1 which may relieve an inhibitory constraint on mTORC1 activity (Wiza et al., 2012). Phosphorylation of PRAS40 by Akt and association with 14-3-3 has also been indicated to be crucial for insulin to stimulate mTOR. (Vander Haar et al., 2007). The primary function of PRAS40 in vivo in Drosophila has been shown to regulate TORC1 activity, and not to act as a downstream target and effector of TORC1 (Pallares et al., 2012).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P03629 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
Click image to see more details
Western blot of Drosophila lysate showing specific immunolabeling of the ~60 kDa PRAS40 protein phosphorylated at Thr356 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 minutes).
Protein Target Info & Infographic
Gene/Protein Information For AKT1S1 (Source: Uniprot.org, NCBI)
Gene Name
AKT1S1
Full Name
Proline-rich AKT1 substrate 1
Weight
27383 MW
Alternative Names
40 kDa proline rich AKT substrate antibody, 40 kDa proline-rich AKT substrate antibody, AKT1 S1 antibody, AKT1 substrate 1 (proline rich) antibody, AKT1 substrate 1 antibody, AKT1S 1 antibody, AKT1S1 antibody, AKTS1_HUMAN antibody, Lobe antibody, MGC2865 antibody, PRAS 40 antibody, PRAS antibody, PRAS40 antibody, Proline rich akt substrate antibody, Proline rich Akt substrate 40 kDa antibody, Proline-rich AKT1 substrate 1 antibody Akt1s1|1110012J22Rik, AI227026, Lo, Lobe, Lobel, PR, PRAS40|AKT1 substrate 1 (proline-rich)|proline-rich AKT1 substrate 1|proline-rich AKT substrate
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKT1S1, check out the AKT1S1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKT1S1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Thr356 PRAS40 Antibody, Phospho-Specific (P03629)
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No publications found for P03629
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Customer Q&As
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6 Customer Q&As for Anti-Phospho-Thr356 PRAS40 Antibody, Phospho-Specific
Question
Will anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific P03629 work on bovine WB with brain {eco:0000312?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
Our lab technicians have not validated anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific P03629 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific P03629 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain {eco:0000312 in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-23
Question
Will P03629 anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
As indicated on the product datasheet, P03629 anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-04
Question
I see that the anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific P03629 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-15
Question
Does anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific P03629 work for WB with colon carcinoma?
Verified Customer
Verified customer
Asked: 2018-10-26
Answer
According to the expression profile of colon carcinoma, AKT1S1 is highly expressed in colon carcinoma. So, it is likely that anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific P03629 will work for WB with colon carcinoma.
Boster Scientific Support
Answered: 2018-10-26
Question
We are currently using anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific P03629 for drosophila tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says drosophila. Is it likely that the antibody can work on zebrafish tissues as well?
M. Krishna
Verified customer
Asked: 2016-01-08
Answer
The anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific (P03629) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-01-08
Question
I was wanting to use your anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific for WB for drosophila colon carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for drosophila colon carcinoma identification?
J. Collins
Verified customer
Asked: 2014-03-21
Answer
It shows on the product datasheet, P03629 anti-Phospho-Thr356 PRAS40 antibody, Phospho-Specific has been tested for WB on drosophila tissues. We have an innovator award program that if you test this antibody and show it works in drosophila colon carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-03-21