Anti-PHOX2A Antibody

Product Name

Anti-PHOX2A Antibody

View all PHOX2A Antibodies

SKU/Catalog Number

A05780

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PHOX2A Antibody (Catalog # A05780). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

PHOX2A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-PHOX2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05780)

Host

Rabbit

Contents

PHOX2A Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

PHOX2A antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human PHOX2A. The immunogen is located within the last 50 amino acids of PHOX2A.

Cross-reactivity

PHOX2A antibody is predicted to not cross-react with other paired homeobox family members.

Reactive Species

A05780 is reactive to PHOX2A in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

29.7 kDa

Background of PHOX2A

PHOX2A and PHOX2B (Paired mesoderm homeobox protein) are closely related, paired-homeodomain transcription factors that function as determinants of the noradrenergic phenotype during embryogenesis. PHOX2 proteins are crucial for the regulation of endogenous hydroxylases in neural crest cells and promote sympathetic neuron generation. Human PHOX2B contains one DNA binding homeobox domain and is required for the differentiation of all central and nonperipheral noradrenergic centers in the brain. In contrast, PHOX2A controls only the differentiation of the main noradrenergic center of the brain. Regulation of PHOX2 may have therapeutic utility in aging or disorders involving degeneration of noradrenergic neurons.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05780 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PHOX2A antibody can be used for detection of PHOX2A by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information PHOX2A

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