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Product Info Summary
| SKU: | M06707 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PI 3 Kinase p55 gamma PIK3R3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M06707
BM4344 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PI 3 Kinase p55 gamma PIK3R3 Rabbit Monoclonal Antibody catalog # M06707. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PI 3 Kinase p55 gamma PIK3R3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06707)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DHC-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PI 3 Kinase p55 gamma
Reactive Species
M06707 is reactive to PIK3R3 in Human, Mouse, Rat
Observed Molecular Weight
50 kDa
Calculated molecular weight
54.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06707 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB:human SH-SY5Y whole cell, human Raji whole cell, human Daudi whole cell, rat brain tissue, mouse brain tissueIHC: Human pituitary tumor tissue, Human lung adenocarcinoma tissue, Human pituitary tumor tissue, human astrocytoma tissue
ICC/IF: C6 cell
Validation Images & Assay Conditions
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Western blot analysis of PIK3R3 using anti-PIK3R3 antibody (M06707).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human Daudi whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R3 antigen affinity purified monoclonal antibody (M06707) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PIK3R3 at approximately 50 kDa. The expected band size for PIK3R3 is at 50 kDa.
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Determination of the relative gene expression. The levels of PIK3R3, AKT2, TGF-β1, E-cadherin, MMP2, TIMP2, BAD, BCL-2, and COLI were detected by qPCR after myopia induction. Significance refers to comparisons of the LIM group to the NC group (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). N = 3.
Index in PubMed under a CC BY license. PMID: 40216914
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The relevant protein expression measured by western blot analysis. After 2- and 4-week myopia induction, expression of PIK3R3 and p-AKT2 ( A ), BAD and BCL-2 ( B ), TGF-β1 and E-cadherin ( C ), and COLI ( D ) in the sclera of the guinea pigs in the NC and LIM groups were detected by western blot analysis. Significance refers to comparisons of the LIM group to the NC group (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). N = 4.
Index in PubMed under a CC BY license. PMID: 40216914
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Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:800 dilution.
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Immunofluorescent analysis of C6 cells, using PI 3 Kinase p55 gamma Antibody .
Specific Publications For Anti-PI 3 Kinase p55 gamma PIK3R3 Rabbit Monoclonal Antibody (M06707)
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3 Customer Q&As for Anti-PI 3 Kinase p55 gamma PIK3R3 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-PI 3 Kinase p55 gamma Rabbit Monoclonal antibody (M06707)?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
We do provide the blocking peptide for product anti-PI 3 Kinase p55 gamma Rabbit Monoclonal antibody (M06707). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-18
Question
Would anti-PI 3 Kinase p55 gamma Rabbit Monoclonal antibody M06707 work on bovine WB with nasal cavity epithelium?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
Our lab technicians have not tested anti-PI 3 Kinase p55 gamma Rabbit Monoclonal antibody M06707 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-PI 3 Kinase p55 gamma Rabbit Monoclonal antibody M06707 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine nasal cavity epithelium in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-22
Question
We are currently using anti-PI 3 Kinase p55 gamma Rabbit Monoclonal antibody M06707 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
T. Banerjee
Verified customer
Asked: 2015-04-16
Answer
The anti-PI 3 Kinase p55 gamma Rabbit Monoclonal antibody (M06707) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-16
