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- Table of Contents
PIK3CG antibody (A01517-2) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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Flow Cytometry analysis of Raji cells using anti-PI3K-gamma/PIK3CG antibody (A01517-2).
Overlay histogram showing Raji cells stained with A01517-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PI3K-gamma/PIK3CG Antibody (A01517-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Raji cells stained with A01517-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PI3K-gamma/PIK3CG Antibody (A01517-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-PI3K-gamma/PIK3CG Antibody Picoband®
PIK3CG antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
PIK3CG antibody was positively detected by WB in:
human K562 whole cell, human Raji whole cell, human Jurkat whole cell, human HepG2 whole cell, rat RH35 whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell, mouse RAW2647 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PIK3CG antibody was positively detected by IHC in:
human breast cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PIK3CG antibody was positively detected by FC in:
Raji cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-PI3K-gamma/PIK3CG Antibody Picoband®
View all PIK3CG Antibodies
View all PIK3CG Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PI3K-gamma/PIK3CG recombinant protein (Position: K52-D308).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
126 kDa
Calculated Molecular Weight
126.5 kDa
Background of PIK3CG
Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform is an enzyme that in humans is encoded by the PIK3CG gene. Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene.
Cite This Product
Anti-PI3K-gamma/PIK3CG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01517-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Cow
93%
High Homology
Sheep
93%
High Homology
Rabbit
92%
High Homology
Rat
89%
Moderate Homology
Mouse
88%
Moderate Homology
Dog
84%
Moderate Homology
Zebrafish
61%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PIK3CG antibody
Immunohistochemistry (IHC) protocol for PIK3CG antibody
Flow Cytometry (FC) protocol for PIK3CG antibody
Publications
Application:
Species:
Establishment of diabetes mellitus model using Bombyx mori silkworms in a low-temperature environment
Published: 2024-01-24
Journal: ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
Application: WB
Impact Factor: 1.50
Species: Silkworm
PMID: 38288495
A comparative study of Mesenchymal Stem Cells transplantation approach to antagonize age-associated ovarian hypofunction with consideration of safety and efficiency
Published: 2021-09-06
Journal: Journal of Advanced Research
Application: WB
Impact Factor: 10.48
Species: Mouse
PMID: 35572405
Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway
Published: 2021-02-24
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 2.95
Species: Rat
PMID: 33649809
Mechanism of Action of Xiaoyao San in Treatment of Ischemic Stroke is Related to Anti-Apoptosis and Activation of PI3K/Akt Pathway
Published: 2021-02-22
Journal: Drug Design Development and Therapy
Application: WB
Impact Factor: 4.16
Species: Human
PMID: 33654381
Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells
Published: 2021-01-26
Journal: ENVIRONMENTAL POLLUTION
Application: WB
Impact Factor: 8.07
Species: Mouse
PMID: 33588191
Optimized integration of fluoxetine and 7, 8-dihydroxyflavone as an efficient therapy for reversing depressive-like behavior in mice during the perimenopausal period
Published: 2020-04-01
Journal: PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY
Application: WB
Impact Factor: 4.36
Species: Mouse
PMID: 32243998
RhoA/Rock2/Limk1/cofilin1 pathway is involved in attenuation of neuronal dendritic spine loss by paeonol in the frontal cortex of D-galactose and aluminum‑induced Alzheimer's disease‑like rat model.
Published: 2020-01-01
Journal: ACTA NEUROBIOLOGIAE EXPERIMENTALIS
Application: WB
Impact Factor: 1.54
Species: Rat
PMID: 32990282
Avβ3 single-stranded DNA aptamer attenuates vascular restenosis via Ras-PI3K/MAPK pathway in rats after percutaneous transluminal coronary angioplasty
Published: 2019-12-26
Journal: ARTIFICIAL ORGANS
Application: WB
Impact Factor: 2.38
Species: Rat
PMID: 31879964
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Product Promise
We promise Anti-PI3K-gamma/PIK3CG Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01517-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026