Anti-PI4K a, type I PIP5K1A Antibody

PIP5K1 alpha antibody

Boster Bio Anti-PI4K a, type I PIP5K1A Antibody (Catalog # A07416). Tested in IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: A07416
Size: 100ug
Reactive Species: Human
Host: Rabbit
Application: IHC

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Product Name

Anti-PI4K a, type I PIP5K1A Antibody

View all PIP5K1 alpha Antibodies

SKU/Catalog Number

A07416

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-PI4K a, type I PIP5K1A Antibody (Catalog # A07416). Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-PI4K a, type I PIP5K1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07416)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-PI4K, type I alpha affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PI4K, type I alpha protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A07416 is reactive to PIP5K1A in Human

Applications

A07416 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

62.633kDa

Background of PIP5K1 alpha

Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha is a member of PI3/PI4-kinase family, PI4K alpha is expressed with the highest amount of expression in the heart, placenta, skeletal muscle, kidney and pancreas. This gene encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Anti-PI4K alpha antibody is ideal for investigators interested in Kinase and Phosphatase Antibodies.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1: 10,000
IHC: 5 µg/mL
WB: 1ug/ml

Validation Images & Assay Conditions

Gene/Protein Information For PIP5K1A (Source: Uniprot.org, NCBI)

Gene Name

PIP5K1A

Full Name

Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha

Weight

62.633kDa

Alternative Names

68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha; EC 2.7.1; EC 2.7.1.68; Phosphatidylinositol-4-phosphate 5-kinase type I alpha; phosphatidylinositol-4-phosphate 5-kinase type-1 alpha; phosphatidylinositol-4-phosphate 5-kinase, type I, alpha; PIP5K1-alpha; PIP5KIalpha; PtdIns(4)P-5-kinase 1 alpha PIP5K1A phosphatidylinositol-4-phosphate 5-kinase type 1 alpha phosphatidylinositol 4-phosphate 5-kinase type-1 alpha|68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha|PIP5K1-alpha|PIP5KIalpha|phosphatidylinositol 4-phosphate 5-kinase type I alpha|ptdIns(4)P-5-kinase 1 alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PIP5K1A, check out the PIP5K1A Infographic

PIP5K1A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PIP5K1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-PI4K a, type I PIP5K1A Antibody

Question

We are currently using anti-PI4K a, type I antibody A07416 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

The anti-PI4K a, type I antibody (A07416) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-09

Question

Would A07416 anti-PI4K a, type I antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

As indicated on the product datasheet, A07416 anti-PI4K a, type I antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-20

Question

I am interested in to test anti-PI4K a, type I antibody A07416 on human hippocampus for research purposes, then I may be interested in using anti-PI4K a, type I antibody A07416 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

The products we sell, including anti-PI4K a, type I antibody A07416, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-07

Question

Does anti-PI4K a, type I antibody A07416 work for IHC with hippocampus?

Verified Customer

Verified customer

Asked: 2017-10-02

Answer

According to the expression profile of hippocampus, PIP5K1A is highly expressed in hippocampus. So, it is likely that anti-PI4K a, type I antibody A07416 will work for IHC with hippocampus.

Boster Scientific Support

Answered: 2017-10-02

Question

Is a blocking peptide available for product anti-PI4K a, type I antibody (A07416)?

Verified Customer

Verified customer

Asked: 2017-09-21

Answer

We do provide the blocking peptide for product anti-PI4K a, type I antibody (A07416). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-09-21

Order DetailsPrice
A07416

100 μg

$599

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