Product Info Summary
| SKU: | A07416 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-PI4K a, type I PIP5K1A Antibody
SKU/Catalog Number
A07416
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-PI4K a, type I PIP5K1A Antibody (Catalog # A07416). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-PI4K a, type I PIP5K1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07416)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-PI4K, type I alpha affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PI4K, type I alpha protein.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A07416 is reactive to PIP5K1A in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
62.6 kDa
Background of PIP5K1A
Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha is a member of PI3/PI4-kinase family, PI4K alpha is expressed with the highest amount of expression in the heart, placenta, skeletal muscle, kidney and pancreas. This gene encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Anti-PI4K alpha antibody is ideal for investigators interested in Kinase and Phosphatase Antibodies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07416 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1: 10,000
IHC: 5 µg/mL
WB: 1ug/ml
Anti-PI4K alpha antibody is tested by IHC and ELISA useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~62.6kDa corresponding to the appropriate cell lysate or extract.
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry of Rabbit anti-PIP5K1A antibody. Tissue: Tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: PIP5K1A antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: PIP5K1A as precipitated red signal with hematoxylin purple nuclear counterstain.
Specific Publications For Anti-PI4K a, type I PIP5K1A Antibody (A07416)
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Customer Q&As
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5 Customer Q&As for Anti-PI4K a, type I PIP5K1A Antibody
Question
We are currently using anti-PI4K a, type I antibody A07416 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The anti-PI4K a, type I antibody (A07416) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-09
Question
Would A07416 anti-PI4K a, type I antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
As indicated on the product datasheet, A07416 anti-PI4K a, type I antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-20
Question
I am interested in to test anti-PI4K a, type I antibody A07416 on human hippocampus for research purposes, then I may be interested in using anti-PI4K a, type I antibody A07416 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
The products we sell, including anti-PI4K a, type I antibody A07416, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-07
Question
Does anti-PI4K a, type I antibody A07416 work for IHC with hippocampus?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
According to the expression profile of hippocampus, PIP5K1A is highly expressed in hippocampus. So, it is likely that anti-PI4K a, type I antibody A07416 will work for IHC with hippocampus.
Boster Scientific Support
Answered: 2017-10-02
Question
Is a blocking peptide available for product anti-PI4K a, type I antibody (A07416)?
Verified Customer
Verified customer
Asked: 2017-09-21
Answer
We do provide the blocking peptide for product anti-PI4K a, type I antibody (A07416). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-09-21

