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Product Info Summary
| SKU: | A00743T638-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PKC Alpha (Phospho-T638) PRKCA Antibody
SKU/Catalog Number
A00743T638-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PKC Alpha (Phospho-T638) PRKCA Antibody catalog # A00743T638-1. Tested in WB,ICC/IF,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKC Alpha (Phospho-T638) PRKCA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00743T638-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human MOR-1 around the phosphorylation site of S375.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00743T638-1 is reactive to PRKCA in Human, Mouse, Rat
Applications
A00743T638-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
76.75kDa
Background of PKC alpha
Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
Ana V. Miletic, J. Biol. Chem., Dec 2006; 281: 38257 - 38265.
Christopher C. Stebbins, Mol. Cell. Biol., Sep 2003; 23: 6291 - 6299.
Vered Schapira, Cancer Res., Jun 2006; 66: 6183 - 6191.
Maya Groysman, J. Biol. Chem., Dec 2002; 277: 50121 - 50130.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:5,000
ICC: 1:50-1:500
IHC: 1:50-1:500
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of PRKCA using Anti-PKC Alpha (Phospho-T638) PRKCA Antibody (A00743T638-1).
ICC staining Phospho-PKC alpha(T638) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-PKC Alpha (Phospho-T638) PRKCA Antibody A00743T638-1
Western blot analysis of Phospho-PKC alpha(T638) on different lysates using anti-Phospho-PKC alpha(T638) antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For PRKCA (Source: Uniprot.org, NCBI)
Gene Name
PRKCA
Full Name
Protein kinase C alpha type
Weight
76.75kDa
Superfamily
protein kinase superfamily
Alternative Names
AAG6; aging-associated gene 6; EC 2.7.11; EC 2.7.11.13; MGC129901; PKC alpha; PKCA; PKC-A; PKC-alpha; PKCAMGC129900; PRKACA; PRKCA; protein kinase C alpha type; protein kinase C, alpha PRKCA AAG6, PKC-alpha, PKCA, PKCI+/-, PKCalpha, PRKACA protein kinase C alpha protein kinase C alpha type|PKC-A|aging-associated gene 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKCA, check out the PRKCA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKC Alpha (Phospho-T638) PRKCA Antibody (A00743T638-1)
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No publications found for A00743T638-1
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