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Product Info Summary
| SKU: | M01940 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PKC beta 2 PRKCB Rabbit Monoclonal Antibody
SKU/Catalog Number
M01940
BM4261 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PKC beta 2 PRKCB Rabbit Monoclonal Antibody catalog # M01940. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKC beta 2 PRKCB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01940)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DOC-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PKC beta 2
Reactive Species
M01940 is reactive to PRKCB in Human, Mouse, Rat
Observed Molecular Weight
77 kDa
Calculated molecular weight
76.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01940 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
Positive Control
WB: human K562 whole cell, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of PRKCB using anti-PRKCB antibody (M01940).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKCB antigen affinity purified monoclonal antibody (Catalog # M01940) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKCB at approximately 77 kDa. The expected band size for PRKCB is at 77 kDa.
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Iodixanol triggered PKC phosphorylation, NOX activation, and eNOS dysregulation in HUVECs. HUVECs were incubated with iodixanol (30 mg I/ml) for 0, 5, 15, 30, 60, or 120 min. The phosphorylation of PKC beta II, p47, Rac1, eNOS (Thr495), and eNOS (Ser1177) was assayed by Western blot analysis (A-E) .
Index in PubMed under a CC BY license. PMID: 33658920
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D-4F inhibited iodixanol-induced NOX activation and eNOS dysregulation through the AMPK/PKC pathway in HUVECs. HUVECs were preincubated in the absence or presence of Compound C (2 μmol/L) for 1 h. Then, cells were incubated with or without D-4F (20 μg/ml) for 8 h and treated with iodixanol (30 mg I/ml) for 30 min. The phosphorylation of PKC beta II, p47, Rac1, and eNOS (Thr495) was assayed by Western blot analysis (A-D) .
Index in PubMed under a CC BY license. PMID: 33658920
Specific Publications For Anti-PKC beta 2 PRKCB Rabbit Monoclonal Antibody (M01940)
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4 Customer Q&As for Anti-PKC beta 2 PRKCB Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-PKC beta 2 Rabbit Monoclonal antibody for IP for human platelet on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human platelet identification?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
You can see on the product datasheet, M01940 anti-PKC beta 2 Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human platelet in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-13
Question
Would anti-PKC beta 2 Rabbit Monoclonal antibody M01940 work on zebrafish WB with putamen?
T. Williams
Verified customer
Asked: 2019-06-26
Answer
Our lab technicians have not validated anti-PKC beta 2 Rabbit Monoclonal antibody M01940 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-PKC beta 2 Rabbit Monoclonal antibody M01940 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish putamen in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-26
Question
Is there a BSA free version of anti-PKC beta 2 Rabbit Monoclonal antibody M01940 available?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PKC beta 2 Rabbit Monoclonal antibody M01940 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-07
Question
Would M01940 anti-PKC beta 2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Brown
Verified customer
Asked: 2013-10-31
Answer
As indicated on the product datasheet, M01940 anti-PKC beta 2 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-10-31
