Anti-PKC delta/PRKCD Antibody

Product Name

Anti-PKC delta/PRKCD Antibody

View all PKC delta Antibodies

SKU/Catalog Number

RP1058

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PKC delta/PRKCD Antibody catalog # RP1058. Tested in IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PKC delta/PRKCD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1058)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PKC delta recombinant protein (Position: M1-E160). Human PKC delta shares 95% and 93% amino acid (aa) sequence identity with mouse and rat PKC delta, respctively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1058 is reactive to PRKCD in Human

Applications

RP1058 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

77 kDa

Calculated molecular weight

77.505kDa

Background of PKC delta

Protein kinase C delta type, also called PKCD, is an enzyme that in humans is encoded by the PRKCD gene. The PRKCD gene encodes a member of the protein kinase C family, members of which are critical for regulation of cell survival, proliferation, and apoptosis. This gene is mapped to 3p21.1. Studies both in human and mice demonstrate that this kinase is involved inB cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. PRKCD is also regulated by phosphorylation on various tyrosine residues including Y311 (by SRC). It has been showed that PRKCD phosphorylates NLRC4 and that this phosphorylation is critical for inflammasome assembly. What’s more, it is also a part of a noncanonical WNT signaling cascade.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

nti-PKC delta antibody, RP1058, Western blotting
All lanes: Anti PKC delta (RP1058) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: CEM Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Predicted bind size: 77KD
Observed bind size: 77KD

Click image to see more details

Figure 2. IF analysis of PKC delta using anti-PKC delta antibody (RP1058).
PKC delta was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PKC delta Antibody (RP1058) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For PRKCD (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.11; EC 2.7.11.13; MAY1; MGC49908; nPKC-delta; PKC delta; PKCD; PKCy; PRKCD; protein kinase C delta type; protein kinase C delta VIII; protein kinase C, delta PRKCD ALPS3, CVID9, MAY1, PKCD, nPKC-delta protein kinase C delta protein kinase C delta type|protein kinase C delta VIII|tyrosine-protein kinase PRKCD

For more info on PRKCD, check out the PRKCD Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRKCD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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