Anti-PKC epsilon/PRKCE Antibody Picoband™

Product Name

Anti-PKC epsilon/PRKCE Antibody Picoband™

View all PKC epsilon Antibodies

SKU/Catalog Number

PB9320

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PKC epsilon/PRKCE Antibody Picoband™ catalog # PB9320. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PKC epsilon/PRKCE Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9320)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PKC epsilon recombinant protein (Position: Q53-R236). Human PKC epsilon shares 99% amino acid (aa) sequence identity with both mouse and rat PKC epsilon.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9320 is reactive to PRKCE in Human, Mouse, Rat

Applications

PB9320 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

88 kDa

Calculated molecular weight

83.674kDa

Background of PKC epsilon

Protein kinase C epsilon type, also known as PKCE, is an enzyme that in humans is encoded by the PRKCE gene. The protein encoded by this gene is one of the PKC family members. PRKCE is mapped to 2p21. This kinase has been shown to be involved in many different cellular functions, such as apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. It has been found that activation of PRKCE can induce VR1 channel activity at room temperature in the absence of any other agonist. PRKCE gene plays a role in apoptosis signaling pathways in thyroid cells and it has been indicated that a naturally occurring PRKCE mutant that functions as a dominant negative can block cell death triggered by a variety of stimuli. Expression of PRKCE inhibits chemotherapy-induced caspase-3 activation and apoptosis, thereby leading to cell survival.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Anti-PKC epsilon Picoband antibody, PB9320, Western blotting
All lanes: Anti PKC epsilon (PB9320) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mosue Brain Tissue Lysate at 50ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 88KD
Observed bind size: 88KD

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Anti-PKC epsilon Picoband antibody, PB9320, IHC(P)
IHC(P): Mouse Intestine Tissue

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Anti-PKC epsilon Picoband antibody, PB9320, IHC(P)
IHC(P): Rat Intestine Tissue

Gene/Protein Information For PRKCE (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.11; EC 2.7.11.13; MGC125656; nPKC-epsilon; PKC epsilon; PKCE; PKCEMGC125657; PRKCE; protein kinase C epsilon type; protein kinase C, epsilon PRKCE PKCE, nPKC-epsilon protein kinase C epsilon protein kinase C epsilon type

For more info on PRKCE, check out the PRKCE Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRKCE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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