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- Table of Contents
PLD1 antibody (PB9380) performs well in WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Anti-PLD1 Picoband antibody, PB9380, Western blotting
All lanes: Anti PLD1 (PB9380) at 0.5ug/ml
Lane 1: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 2: 22RV1 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 124KD
Observed bind size: 124KD
All lanes: Anti PLD1 (PB9380) at 0.5ug/ml
Lane 1: Mouse Cardiac Muscle Tissue Lysate at 50ug
Lane 2: 22RV1 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 124KD
Observed bind size: 124KD
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Anti-Phospholipase D1/PLD1 Antibody Picoband®
PLD1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | WB |
Tested Applications
PLD1 antibody was positively detected by WB in:
Mouse Cardiac Muscle Tissue, 22RV1 Whole Cell, HELA Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
Mouse
Tested
Human
Predicted
Monkey
Predicted
Dog
Predicted
Sheep
Predicted
Bovine
Predicted
Rat
Predicted
Product Information
Product Name
Anti-Phospholipase D1/PLD1 Antibody Picoband®
View all PLD1 Antibodies
View all PLD1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PLD1 recombinant protein (Position: M1-H330). Human PLD1 shares 91.8% and 90.9% amino acid (aa) sequence identity with mouse and rat PLD1, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
124 kDa
Calculated Molecular Weight
124.2 kDa
Background of PLD1
By somatic cell hybrid analysis, PLD1 is mapped to 3q26.31. This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. And PLD1 disrupted association of gamma-secretase protein components, independent of PLD1catalytic activity. What's more, PLD1 regulates intracellular trafficking of beta-amyloid, distinct from its effect on gamma-secretase activity.
Cite This Product
Anti-Phospholipase D1/PLD1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9380)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
97%
High Homology
Sheep
95%
High Homology
Cow
93%
High Homology
Mouse
92%
High Homology
Rat
91%
High Homology
Rabbit
79%
Moderate Homology
Zebrafish
62%
Low homology
Drosophila
33%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PLD1 antibody
Publications
Application:
Species:
Study on low concentration deltamethrin toxicity mediated by phospholipase D in Chinese mitten crab (Eriocheir sinensis) ovary
Published: 2024-09-10
Journal: AQUATIC TOXICOLOGY
Application: IF
Impact Factor: 4.10
Species: Eriocheir sinensis
PMID: 39277993
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Product Promise
We promise Anti-Phospholipase D1/PLD1 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9380
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026