Anti-PLEKHB1 Antibody Picoband™

Product Name

Anti-PLEKHB1 Antibody Picoband™

View all PLEKHB1/PHR1 Antibodies

SKU/Catalog Number

A11037-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PLEKHB1 Antibody Picoband™ catalog # A11037-1. Tested in WB, ELISA applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PLEKHB1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11037-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human PLEKHB1 recombinant protein (Position: H82-S214). Human PLEKHB1 shares 93.9% and 92.4% amino acid (aa) sequence identity with mouse and rat PLEKHB1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A11037-1 is reactive to Plekhb1 in Human, Monkey, Mouse, Rat

Applications

A11037-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

27 kDa

Calculated molecular weight

27.34kDa

Background of PLEKHB1/PHR1

PLEKHB1, also named as Evectin-1, EVT1, KPL1, PHR1 and PHRET1, isrequired for properlocalization of retinogeniculate projections but not for eye-specific segregation. PLEKHB1 has 4 isoforms with MW from 21-27kd. It has Dimerform withMW42-54 kDa.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Monkey, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PLEKHB1 using anti-PLEKHB1 antibody (A11037-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: monkey RF/6A whole cell lysates,
Lane 5: rat eye tissue lysates,
Lane 6: mouse eye tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLEKHB1 antigen affinity purified polyclonal antibody (Catalog # A11037-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLEKHB1 at approximately 27 kDa. The expected band size for PLEKHB1 is at 27 kDa.

Gene/Protein Information For Plekhb1 (Source: Uniprot.org, NCBI)

Alternative Names

Evectin-1; EVT1; KPL1evectin-1; PH domain containing, retinal 1; PH domain-containing family B member 1; PH domain-containing protein in retina 1; PHR1PH domain containing protein in retina 1; PHRET1; pleckstrin homology domain containing, family B (evectins) member 1; Pleckstrin homology domain retinal protein 1; pleckstrin homology domain-containing family B member 1 Plekhb1|AI315628, PH, PHR1, Phre, Phret1, evt-, evt-1|pleckstrin homology domain containing, family B (evectins) member 1|pleckstrin homology domain-containing family B member 1|PH domain-containing family B member 1|PH domain-containing protein in retina 1|evectin-1|pleckstrin homology domain retinal protein 1

For more info on Plekhb1, check out the Plekhb1 Infographic

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In this infographic, you will see the following information for Plekhb1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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