Anti-Plunc/BPIFA1 Antibody Picoband™

Product Name

Anti-Plunc/BPIFA1 Antibody Picoband™

View all PLUNC Antibodies

SKU/Catalog Number

A03162-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Plunc/BPIFA1 Antibody Picoband™ catalog # A03162-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Plunc/BPIFA1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03162-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Plunc/BPIFA1 recombinant protein (Position: L64-V256).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03162-2 is reactive to Bpifa1 in Human, Mouse

Applications

A03162-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

27 kDa

Calculated molecular weight

28.625kDa

Background of PLUNC

Palate, lung, and nasal epithelium clone protein[5] (PLUNC) is a gene encoding a secretory protein. This gene is the human homolog of murine plunc, and like the mouse gene, is specifically expressed in the upper airways and nasopharyngeal regions. The encoded antimicrobial protein displays antibacterial activity against Gram-negative bacteria. It is thought to be involved in inflammatory responses to irritants in the upper airways and may also serve as a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer. Multiple transcript variants resulting from alternative splicing in the 3' UTR have been detected, but the full-length nature of only three are known.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Mouse
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Plunc/BPIFA1 using anti-Plunc/BPIFA1 antibody (A03162-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plunc/BPIFA1 antigen affinity purified polyclonal antibody (Catalog # A03162-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plunc/BPIFA1 at approximately 27 kDa. The expected band size for Plunc/BPIFA1 is at 27 kDa.

Gene/Protein Information For Bpifa1 (Source: Uniprot.org, NCBI)

Alternative Names

bA49G10.5; BPIFA1; ligand-binding protein RYA3; LPLUNC3; Lung-specific protein X; LUNX; LUNXNASG; Nasopharyngeal carcinoma-related protein; Palate lung and nasal epithelium clone protein; palate, lung and nasal epithelium associated; palate, lung and nasal epithelium carcinoma associated; PLUNC; protein Plunc; Secretory protein in upper respiratory tracts; SPLUNC1; SPLUNC1SPURT; tracheal epithelium enriched protein; Tracheal epithelium-enriched protein; Von Ebner protein Hl Bpifa1|LUNX, NASG, Pl, Plunc, SPL, SPLUNC1, SPURT|BPI fold containing family A, member 1|BPI fold-containing family A member 1|palate lung and nasal epithelium clone protein|palate, lung, and nasal epithelium associated|palate, lung, and nasal epithelium clone|palate, lung, and nasal epithelium expressed transcript|protein Plunc

For more info on Bpifa1, check out the Bpifa1 Infographic

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In this infographic, you will see the following information for Bpifa1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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