Product Info Summary
SKU: | M03359 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human, Rabbit, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IP, IF, IHC, WB |
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Product info
Product Name
Anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal Antibody
SKU/Catalog Number
M03359
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal Antibody (Catalog # M03359). Tested in Flow Cytometry, IF, IP, WB, IHC applications. This antibody reacts with Human, Rabbit, Rat.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03359)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: 3D3
Isotype
IgG1, kappa
Immunogen
A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M03359 is reactive to PODXL in Human, Rabbit, Rat
Applications
M03359 is guaranteed for Flow Cytometry, IP, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
58.635kDa
Background of PODXL
Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunoprecipitation (1-2ug/500ug protein)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Anti-Podocalyxin Mouse Monoclonal Antibody (3D3).
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Western Blot analysis of HeLa cell lysate using Anti-Podocalyxin Mouse Monoclonal Antibody (3D3).
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Formalin-fixed, paraffin-embedded human Placenta stained with Anti-Podocalyxin Mouse Monoclonal Antibody (3D3).
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Confocal Immunofluorescence of HeLa cells using Anti-Podocalyxin Mouse Monoclonal Antibody (3D3) labeled with CF488 (Green); Reddot is used to label the nuclei.
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Flow Cytometry of NCCIT cells using Anti-Podocalyxin Mouse Monoclonal Antibody (3D3).
Protein Target Info & Infographic
Gene/Protein Information For PODXL (Source: Uniprot.org, NCBI)
Gene Name
PODXL
Full Name
Podocalyxin
Weight
58.635kDa
Superfamily
podocalyxin family
Alternative Names
GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL PODXL Gp200, PC, PCLP, PCLP-1, PDX1, gp135, PODXL podocalyxin like podocalyxin|GCTM-2 antigen|podocalyxin-like protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PODXL, check out the PODXL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PODXL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal Antibody (M03359)
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No publications found for M03359
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal Antibody
Question
Is a blocking peptide available for product anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal antibody (M03359)?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
We do provide the blocking peptide for product anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal antibody (M03359). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-09
Question
Will anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal antibody M03359 work for IP with heart?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
According to the expression profile of heart, PODXL is highly expressed in heart. So, it is likely that anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal antibody M03359 will work for IP with heart.
Boster Scientific Support
Answered: 2019-12-16
Question
Is there a BSA free version of anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal antibody M03359 available?
B. Lewis
Verified customer
Asked: 2013-02-21
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal antibody M03359 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-02-21