Anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal Antibody

PODXL antibody

Boster Bio Anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal Antibody (Catalog # M03359). Tested in Flow Cytometry, IF, IP, WB, IHC applications. This antibody reacts with Human, Rabbit, Rat.

Product Info Summary

SKU: M03359
Size: 100ug/vial
Reactive Species: Human, Rabbit, Rat
Host: Mouse
Application: Flow Cytometry, IP, IF, IHC, WB

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Product Name

Anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal Antibody

View all PODXL Antibodies

SKU/Catalog Number

M03359

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal Antibody (Catalog # M03359). Tested in Flow Cytometry, IF, IP, WB, IHC applications. This antibody reacts with Human, Rabbit, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03359)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: 3D3

Isotype

IgG1, kappa

Immunogen

A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M03359 is reactive to PODXL in Human, Rabbit, Rat

Applications

M03359 is guaranteed for Flow Cytometry, IP, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

58.635kDa

Background of PODXL

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunoprecipitation (1-2ug/500ug protein)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For PODXL (Source: Uniprot.org, NCBI)

Gene Name

PODXL

Full Name

Podocalyxin

Weight

58.635kDa

Superfamily

podocalyxin family

Alternative Names

GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL PODXL Gp200, PC, PCLP, PCLP-1, PDX1, gp135, PODXL podocalyxin like podocalyxin|GCTM-2 antigen|podocalyxin-like protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PODXL, check out the PODXL Infographic

PODXL infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PODXL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal Antibody

Question

Is a blocking peptide available for product anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal antibody (M03359)?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

We do provide the blocking peptide for product anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal antibody (M03359). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-09

Question

Will anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal antibody M03359 work for IP with heart?

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

According to the expression profile of heart, PODXL is highly expressed in heart. So, it is likely that anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal antibody M03359 will work for IP with heart.

Boster Scientific Support

Answered: 2019-12-16

Question

Is there a BSA free version of anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal antibody M03359 available?

B. Lewis

Verified customer

Asked: 2013-02-21

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Monoclonal antibody M03359 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-02-21

Order DetailsPrice
M03359-100ug

100 μg

$468

Free Secondary Antibody

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M03359
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$468.00

Troubleshooting

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