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Product Info Summary
| SKU: | M00305-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-POMC Monoclonal Antibody
SKU/Catalog Number
M00305-1
BM5411 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-POMC Monoclonal Antibody catalog # M00305-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-POMC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00305-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADEC-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human POMC ACTH stimulates the adrenal glands to release cortisol. MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin and Met-enkephalin are endogenous opiates.
Reactive Species
M00305-1 is reactive to POMC in Human, Mouse, Rat
Observed Molecular Weight
29 kDa
Calculated molecular weight
29.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00305-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Positive Control
WB: mouse pituitary
Validation Images & Assay Conditions
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Western blot analysis of POMC expression in mouse pituitary lysate.
Specific Publications For Anti-POMC Monoclonal Antibody (M00305-1)
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4 Customer Q&As for Anti-POMC Monoclonal Antibody
Question
Our lab used your anti-POMC Monoclonal antibody for WB on pituitary in the past. I am using mouse, and I plan to use the antibody for IP next. We want examining pituitary as well as pituitary gland in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
D. Edwards
Verified customer
Asked: 2020-02-25
Answer
I viewed the website and datasheets of our anti-POMC Monoclonal antibody and it appears that M00305-1 has been tested on mouse in both WB and IP. Thus M00305-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-25
Question
We have seen staining in mouse pituitary gland. Do you have any suggestions? Is anti-POMC Monoclonal antibody supposed to stain pituitary gland positively?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
According to literature pituitary gland does express POMC. According to Uniprot.org, POMC is expressed in pituitary gland, pituitary, among other tissues. Regarding which tissues have POMC expression, here are a few articles citing expression in various tissues:
Pituitary, Pubmed ID: 7828531, 15489334, 16807684
Boster Scientific Support
Answered: 2019-10-18
Question
We are currently using anti-POMC Monoclonal antibody M00305-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-03
Answer
The anti-POMC Monoclonal antibody (M00305-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-03
Question
We were satisfied with the WB result of your anti-POMC Monoclonal antibody. However we have been able to see positive staining in pituitary gland secreted. using this antibody. Is that expected? Could you tell me where is POMC supposed to be expressed?
K. Singh
Verified customer
Asked: 2017-07-24
Answer
From what I have seen in literature, pituitary gland does express POMC. Generally POMC expresses in secreted. Regarding which tissues have POMC expression, here are a few articles citing expression in various tissues:
Pituitary, Pubmed ID: 7828531, 15489334, 16807684
Boster Scientific Support
Answered: 2017-07-24
