Product Info Summary
SKU: | A00449S112 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-PPAR Gamma (phospho-S112) PPARG Antibody
View all PPAR gamma/NR1C3 Antibodies
SKU/Catalog Number
A00449S112
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PPAR Gamma (phospho-S112) PPARG Antibody catalog # A00449S112. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PPAR Gamma (phospho-S112) PPARG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00449S112)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Insulin R around the phosphorylation site of Y1361.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00449S112 is reactive to PPARG in Human, Mouse, Rat
Applications
A00449S112 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
57.62kDa
Background of PPAR gamma/NR1C3
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Fibroblast Activation Protein (FAP) is expressed in the stroma of sites that are undergoing wound healing. In addition, it has recently been reported that FAP is expressed in the stroma of sites of metastatic disease. Inhibition of FAP may lead to a dramatic decrease in the number of metastatic osteosarcoma lung nodules. FAP exists as an inactive monomer and when activated forms homodimers or heterodimers with DPP4. Multiple isoforms of FAP are reported as alternative splicing products from a common gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of PPARG using Anti-PPAR Gamma (phospho-S112) PPARG Antibody (A00449S112).
Immunohistochemistry (IHC) analyzes of p-PPARγ (S112) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-PPAR Gamma (phospho-S112) PPARG Antibody A00449S112
Western blot (WB) analysis of p-PPAR-γ (S112) polyclonal antibody at 1:500 dilutionLane1:Hela cell lysate treated with colchicine
Lane2:Raw264.7 cell lysate treated with colchicine
Lane3:PC12 cell lysate treated with colchicine
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PPARG (Source: Uniprot.org, NCBI)
Gene Name
PPARG
Full Name
Peroxisome proliferator-activated receptor gamma
Weight
57.62kDa
Superfamily
nuclear hormone receptor family
Alternative Names
CIMT1; NR1C3; NR1C3GLM1; Nuclear receptor subfamily 1 group C member 3; peroxisome proliferator-activated receptor gamma 1; peroxisome proliferator-activated receptor gamma; PPAR gamma; PPARG; PPARG1peroxisome proliferative activated receptor, gamma; PPARG2PPARgamma; PPAR-gamma PPARG CIMT1, GLM1, NR1C31, PPARG2, PPARG5, PPARgamma, PPARG peroxisome proliferator activated receptor gamma peroxisome proliferator-activated receptor gamma|PPAR-gamma|nuclear receptor subfamily 1 group C member 3|peroxisome proliferator-activated nuclear receptor gamma variant 1|peroxisome proliferator-activated receptor-gamma 5|peroxisome proliferator-activated receptor-gamma splicing
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPARG, check out the PPARG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPARG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPAR Gamma (phospho-S112) PPARG Antibody (A00449S112)
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No publications found for A00449S112
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