Product Info Summary
SKU: | A14433-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-PPP1R14D Antibody
View all PPP1R14D/GBPI-1 Antibodies
SKU/Catalog Number
A14433-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PPP1R14D Antibody catalog # A14433-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PPP1R14D Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14433-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human PPP1R14D
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14433-1 is reactive to PPP1R14D in Human
Applications
A14433-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
16.508kDa
Background of PPP1R14D/GBPI-1
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms.
Suzuki T., J. Biol. Chem. 276:21724-21736(2001).
Lai C.-H., Genome Res. 10:703-713(2000).
Grimwood J., Nature 428:529-535(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-PPP1R14D Antibody A14433-1
Western Blot (WB) analysis of specific cells using PPP1R14D polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For PPP1R14D (Source: Uniprot.org, NCBI)
Gene Name
PPP1R14D
Full Name
Protein phosphatase 1 regulatory subunit 14D
Weight
16.508kDa
Superfamily
PP1 inhibitor family
Alternative Names
CPI17-like; FLJ20251; gastrointestinal and brain-specific PP1-inhibitory protein 1; GBPI; GBPI1; GBPI-1; gut and brain phosphatase inhibitor 1; MGC119014; MGC119016; PKC-dependent PP1 inhibitory protein; protein phosphatase 1 regulatory subunit 14D; protein phosphatase 1, regulatory (inhibitor) subunit 14D PPP1R14D CPI17-like, GBPI-1, GBPI1 protein phosphatase 1 regulatory inhibitor subunit 14D protein phosphatase 1 regulatory subunit 14D|PKC-dependent PP1 inhibitory protein|gastrointestinal and brain-specific PP1-inhibitory protein 1|gut and brain phosphatase inhibitor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP1R14D, check out the PPP1R14D Infographic
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In this infographic, you will see the following information for PPP1R14D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPP1R14D Antibody (A14433-1)
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No publications found for A14433-1
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