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Product Info Summary
| SKU: | A14433 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-PPP1R14D/Gbpi 1 Antibody
SKU/Catalog Number
A14433
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PPP1R14D/Gbpi 1 Antibody catalog # A14433. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PPP1R14D/Gbpi 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14433)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PPP1R14D. AA range:66-115
Reactive Species
A14433 is reactive to PPP1R14D in Human, Mouse, Rat
Calculated molecular weight
16.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14433 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using PPP1R14D Polyclonal Antibody
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Western blot analysis of lysates from HeLa, HepG2, and COLO cells, using PPP1R14D Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4° overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-PPP1R14D/Gbpi 1 Antibody (A14433)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-PPP1R14D/Gbpi 1 Antibody
Question
Here is the WB image, lot number and protocol we used for mucosa of transverse colon using anti-PPP1R14D/Gbpi 1 antibody A14433. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-23
Question
We are currently using anti-PPP1R14D/Gbpi 1 antibody A14433 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
L. Jones
Verified customer
Asked: 2020-04-08
Answer
The anti-PPP1R14D/Gbpi 1 antibody (A14433) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-08
Question
I have a question about product A14433, anti-PPP1R14D/Gbpi 1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14433 anti-PPP1R14D/Gbpi 1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-26
Question
Does anti-PPP1R14D/Gbpi 1 antibody A14433 work for WB with mucosa of transverse colon?
G. Anderson
Verified customer
Asked: 2018-11-16
Answer
According to the expression profile of mucosa of transverse colon, PPP1R14D is highly expressed in mucosa of transverse colon. So, it is likely that anti-PPP1R14D/Gbpi 1 antibody A14433 will work for WB with mucosa of transverse colon.
Boster Scientific Support
Answered: 2018-11-16
