Anti-PRAME Picoband™ Antibody

Product Name

Anti-PRAME Picoband™ Antibody

View all PRAME Antibodies

SKU/Catalog Number

A06628-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PRAME Picoband™ Antibody catalog # A06628-2. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PRAME Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06628-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PRAME recombinant protein (Position: T21-N509).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A06628-2 is reactive to PRAME in Human

Applications

A06628-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

58 kDa

Calculated molecular weight

57.89kDa

Background of PRAME

Melanoma antigen preferentially expressed in tumors is a protein that in humans is encoded by the PRAME gene. It is mapped to 22q11.22. This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PRAME using anti-PRAME antibody (A06628-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRAME antigen affinity purified polyclonal antibody (Catalog # A06628-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRAME at approximately 58KD. The expected band size for PRAME is at 58KD.

Gene/Protein Information For PRAME (Source: Uniprot.org, NCBI)

Alternative Names

cancer/testis antigen 130; MAPEOIP4CT130; melanoma antigen preferentially expressed in tumors; OIP-4; Opa-interacting protein 4; Opa-interacting protein OIP4; preferentially expressed antigen in melanoma; Preferentially expressed antigen of melanoma PRAME CT130, MAPE, OIP-4, OIP4 PRAME nuclear receptor transcriptional regulator melanoma antigen preferentially expressed in tumors|Opa-interacting protein OIP4|cancer/testis antigen 130|opa-interacting protein 4|preferentially expressed antigen in melanoma|preferentially expressed antigen of melanoma

For more info on PRAME, check out the PRAME Infographic

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In this infographic, you will see the following information for PRAME: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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