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Product Info Summary
| SKU: | A00782 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PRDM16 Antibody
SKU/Catalog Number
A00782
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PRDM16 Antibody (Catalog # A00782). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PRDM16 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PRDM16 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00782)
Host
Rabbit
Contents
PRDM16 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PRDM16 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human PRDM16. The immunogen is located within amino acids 1120 - 1170 of PRDM16.
Reactive Species
A00782 is reactive to PRDM16 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
140.3 kDa
Background of PRDM16
PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36, q21)-positive MDS/AML. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Recent studies have shown that PRDM16 normally acts as a Smad3 binding protein that may be important for the development of orofacial structures through modulation of the TGF-beta signaling pathway. Other experiments have indicated that PRDM16 controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00782 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-4 μg/mL; IHC-P: 1-2 μg/mL; IHC-P/IF: 10-20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse, and rat samples; Immunofluorescence in the mouse sample. All other applications and species not yet tested.
Validation Images & Assay Conditions
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WB Validation in MCF7 Cells
Loading: 15 μg of lysate
Antibodies: PRDM16, A00782, 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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WB Validation in Mouse Lung
Loading: 15 μg of lysate
Antibodies: PRDM16, A00782, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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WB Validation in Rat Spleen
Loading: 15 μg of lysate
Antibodies: PRDM16, A00782, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of PRDM16 in Human Kidney
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRDM16 antibody (A00782) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of PRDM16 in Human Brain
Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-PRDM16 antibody (A00782) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of PRDM16 in Mouse Kidney
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRDM16 antibody (A00782) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of PRDM16 in Mouse Brain
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling PRDM16 with A00782 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Immunohistochemistry Validation of PRDM16 in Rat Spleen
Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-PRDM16 antibody (A00782) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Specific Publications For Anti-PRDM16 Antibody (A00782)
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