Anti-Pre-mRNA-splicing factor SPF27 BCAS2 Antibody

Product Name

Anti-Pre-mRNA-splicing factor SPF27 BCAS2 Antibody

View all BCAS2 Antibodies

SKU/Catalog Number

A07113

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Pre-mRNA-splicing factor SPF27 BCAS2 Antibody (Catalog # A07113). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

BCAS2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Pre-mRNA-splicing factor SPF27 BCAS2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07113)

Host

Rabbit

Contents

BCAS2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

BCAS2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human BCAS2. The immunogen is located within the last 50 amino acids of BCAS2.

Reactive Species

A07113 is reactive to BCAS2 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

26.1 kDa

Background of BCAS2

BCAS2 was identified through differential display analysis as an mRNA species that was overexpressed in MCF7 and BT-20 breast cancer cell lines. The chromosomal region containing this gene, 1p13.3021, is amplified in these cells lines. BCAS2 is a transcriptional cofactor that enhances estrogen receptor-mediated gene expression, and directly interacts with the tumor suppressor p53 to reduce p53 transcriptional activity by reducing p53 protein level in the absence of DNA damage. Deprivation of BCAS2 through RNA inhibition induces apoptosis in p53-wild type cells, but causes G2-M arrest in p53-null or -mutant cells; this effect was reversed with the expression of ectopic BCAS2. BCAS2 may thus be potentially useful as a therapeutic target in the treatment of cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A07113 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

BCAS2 antibody can be used for detection of BCAS2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information BCAS2

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