BCAS2 Antibodies

AND BCAS2 ELISA kits, BCAS2 Proteins

Many biological assays use antibodies to detect BCAS2, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with BCAS2 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop BCAS2 antibodies...

We validate the specificity of these antibodies to BCAS2 by testing them on tissues known to express BCAS2 positively and negatively. Browse below to find the BCAS2 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for BCAS2? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the BCAS2 IHC Guide

BCAS2 Infographic by Boster Bio

All BCAS2 Products

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Specificity

Applications

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Anti-Pre-mRNA-splicing factor SPF27 BCAS2 Antibody

Western blot analysis of BCAS2 in MCF7 cell lysate with BCAS2 antibody at (A) 0

Immunohistochemistry of BCAS2 in human breast carcinoma with BCAS2 antibody at 5 μg/mL

Immunofluorescence of BCAS2 in human breast carcinoma tissue with BCAS2 antibody at 20 μg/mL

Human, Mouse, Rat

ELISA, IF, IHC-P, WB

$481
Cat # A07113
0.1 mg

BCAS2 (NM_005872) Human Over-expression Lysate

Human

$628
Cat # LS010286
20 µg, 100 µg

Anti-Pre-mRNA-splicing factor SPF27 BCAS2 Antibody

Western blot (WB) analysis of BCAS2 polyclonal antibody at 1:500 dilution
Lane1:HEK293T cell lysate
Lane2:Mouse heart tissue lysate
Lane3:PC12 cell lysate

Human, Mouse, Rat

IP, ICC, WB

$405
Cat # A07113-1
100ul

BCAS2 Overview

Facts about Pre-mRNA-splicing factor SPF27.

BCAS2 3D structure. Source: alphafold

Pre-mRNA-splicing factor SPF27 (BCAS2)

Part of the PRP19-CDC5L complex that forms an integral component of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex.

The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR). .

Gene Information

Human
Gene Name:	BCAS2
Uniprot:	O75934
Entrez:	10286

Family

Belongs to:

SPF27 family

Alternative Names

breast carcinoma amplified sequence 2; Breast carcinoma-amplified sequence 2; DAM1DNA amplified in mammary carcinoma 1 protein; pre-mRNA-splicing factor SPF27; Snt309; SPF27; spliceosome associated protein, amplified in breast cancer; Spliceosome-associated protein SPF 27

Molecular Weight

Mass (kDA):
26.131 kDA

Genomic Context


Human

Location:	1p13.2
Sequence:	1; NC_000001.11 (114567557..114581615, complement)

Tissue Specificity

Ubiquitously expressed.

Subcellular Localizations

Nucleus. Nucleus, nucleolus.

Diseases

Malignant Neoplasm Of Breast
Malignant Neoplasms
Mammary Neoplasms
Carcinoma
Neoplasms
Ductal Breast Carcinoma
Stomach Neoplasms
Cell Transformation, Neoplastic
Genital Neoplasms, Female
Endometrial Polyp

Breast Diseases
Carcinoma In Situ
Noninfiltrating Intraductal Carcinoma
Pancreatic Neoplasm
Carcinoma, Lobular

Interacting Proteins

AIMP2
CDC5L
GOLGA2
HGS
IKZF3

KRT34
KRT40
LMO4
PRPF19
PRPF8

SF3B4
TCF4

Pathways

Methylation
Microtubule Depolymerization
Histone H2b Ubiquitination
Histone Modification

PTMs

Methylation

Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/BCAS2

References

PMID: 9731529 by Neubauer G., et al. Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex.

PMID: 10403562 by Nagasaki K., et al. Identification of a novel gene, DAM1, amplified at chromosome 1p13.3- 21 region in human breast cancer cell lines.