Anti-Phospho-Progesterone Receptor (Ser190) PGR Antibody

Product Name

Anti-Phospho-Progesterone Receptor (Ser190) PGR Antibody

View all PGR Antibodies

SKU/Catalog Number

P00541

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Progesterone Receptor (Ser190) PGR Antibody (Catalog # P00541). Tested in WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-Progesterone Receptor (Ser190) PGR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00541)

Host

Mouse

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Monoclonal

Clone Number

1154

Isotype

IgG1

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser190 of human progesterone receptor, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00541 is reactive to PGR in Human

Applications

P00541 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

98.981kDa

Background of PGR

There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer (Edwards, 2005). The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones (Attia et al., 2000; Lin et al., 2003). Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor (Clemm et al., 2000) . Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser-190 and Ser-294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IHC: 1:1000

Validation Images & Assay Conditions

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Western blot of T47D cell lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90 kDa PR-A isoform and the ~120 kDa PR-B isoform of the progesterone receptor phosphorylated at Ser¹⁹⁰. The immunolabeling is specifically blocked by the phosphopeptide used as the antigen (not shown).

Gene/Protein Information For PGR (Source: Uniprot.org, NCBI)

Alternative Names

NR3C3; NR3C3PRNuclear receptor subfamily 3 group C member 3; PGR; Progesterone R; progesterone receptor; ProgesteroneR PGR NR3C3, PR progesterone receptor progesterone receptor|nuclear receptor subfamily 3 group C member 3

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