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- Table of Contents
Prom1 antibody (A01767-4) performs well in ELISA, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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IHC analysis of CD133/Prom1 using anti-CD133/Prom1 antibody (A01767-4).
CD133/Prom1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD133/Prom1 Antibody (A01767-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
CD133/Prom1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD133/Prom1 Antibody (A01767-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Anti-CD133/Prom1 Antibody Picoband®
Prom1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, IHC, WB |
Tested Applications
Prom1 antibody was positively detected by WB in:
mouse kidney tissue, rat kidney tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Prom1 antibody was positively detected by IHC in:
mouse kidney tissue, rat kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse, Rat
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Mouse
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-CD133/Prom1 Antibody Picoband®
View all PROM1 Antibodies
View all PROM1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Prom1 recombinant protein (Position: E20-Y867).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
130 kDa
Calculated Molecular Weight
97.1 kDa
Background of Prom1
Prominin-1, also known as CD133, is a glycoprotein that in humans is encoded by the PROM1 gene. It is mapped to 4p15.32. Prominin-1 is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. It has been proposed to act as an organizer of cell membrane topology. Prominin-1 was expressed not only on metastatic colon cancer cells, but also on differentiated colonic epithelium in both adult mice and humans.
Cite This Product
Anti-CD133/Prom1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01767-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
83%
Moderate Homology
Human
60%
Low homology
Monkey
60%
Low homology
Rabbit
59%
Low homology
Dog
56%
Low homology
Sheep
51%
Low homology
Cow
51%
Low homology
Zebrafish
41%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Prom1 antibody
Immunohistochemistry (IHC) protocol for Prom1 antibody
Publications
Application:
Species:
Genipin stabilized fibrin microbeads: Carrying cytokines to form niches for stem cell differentiation
Published: 2025-05-20
Journal: JOURNAL OF BIOMATERIALS APPLICATIONS
Application: IF
Impact Factor: 2.50
Species: Mouse
PMID: 40391599
Endothelial progenitor cells improve functional recovery in focal cerebral ischemia of rat by promoting angiogenesis via VEGF
Published: 2018-07-21
Journal: JOURNAL OF CLINICAL NEUROSCIENCE
Application: IHC
Impact Factor: 1.64
Species: Rat
PMID: 30041898
1-Methyl-D-tryptophan Reduces Tumor CD133+ cells, Wnt/β-catenin and NF-κβp65 while Enhances Lymphocytes NF-κβ2, STAT3, and STAT4 Pathways in Murine Pancreatic Adenocarcinoma
Published: 2018-06-29
Journal: Scientific Reports
Application: WB
Impact Factor: 4.12
Species: Mouse
PMID: 29959375
Dual Receptor Recognizing Cell Penetrating Peptide for Selective Targeting, Efficient Intratumoral Diffusion and Synthesized Anti-Glioma Therapy
Published: 2016-01-01
Journal: Theranostics
Application: WB
Impact Factor: 8.85
Species: Mouse
PMID: 26877777
Fibroblast growth factor 5-short (FGF5s) inhibits the activity of FGF5 in primary and secondary hair follicle dermal papilla cells of cashmere goats
Published: 2015-09-21
Journal: GENE
Application: IF
Impact Factor: 2.14
Species: Goat
PMID: 26390813
Multifunctional liposomes loaded with paclitaxel and artemether for treatment of invasive brain glioma
Published: 2014-04-13
Journal: BIOMATERIALS
Application: IHC
Impact Factor: 8.31
Species: Rat
PMID: 24726749
Antagonism of Bradykinin B2 Receptor Prevents Inflammatory Responses in Human Endothelial Cells by Quenching the NF-kB Pathway Activation
Published: 2014-01-02
Journal: PLoS One
Application: IHC
Impact Factor: 3.53
Species: Rat
PMID: 24392129
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Product Promise
We promise Anti-CD133/Prom1 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01767-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026