Anti-Proteasome subunit beta type-8 PSMB8 Antibody

Product Name

Anti-Proteasome subunit beta type-8 PSMB8 Antibody

View all LMP7/PSMB8 Antibodies

SKU/Catalog Number

A02188-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Proteasome subunit beta type-8 PSMB8 Antibody catalog # A02188-2. Tested in WB,ICC,IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Proteasome subunit beta type-8 PSMB8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02188-2)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Arrestin-beta-1 around the phosphorylation site of S412.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02188-2 is reactive to PSMB8 in Human, Mouse, Rat

Applications

A02188-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

30.354kDa

Background of LMP7/PSMB8

Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1 and BHLHE40/DEC1, which decreases its transcriptional repression activity. Deconjugates SUMO1 from CLOCK, which decreases its transcriptional activation activity.

Taihei Yamaguchi, Mol. Cell. Biol., Jun 2005; 25: 5171 - 5182.
Jinke Cheng, Mol. Cell. Biol., Jul 2004; 24: 6021 - 6028.
Molly A. Brewer, AACR Meeting Abstracts, Mar 2004; 2004: 1006.
Imke M. Veltman, Hum. Mol. Genet., Jul 2005; 14: 1955 - 1963.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of PSMB8 using Anti-Proteasome subunit beta type-8 PSMB8 Antibody (A02188-2).

ICC staining Proteasome 20S LMP7 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-Proteasome subunit beta type-8 PSMB8 Antibody A02188-2

Western blot analysis of Proteasome 20S LMP7 on U937 (1) and A431 (2) cell lysate using anti-Proteasome 20S LMP7 antibody at 1/500 dilution.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For PSMB8 (Source: Uniprot.org, NCBI)

Alternative Names

ALDD; beta5i; D6S216E; EC 3.4.25.1; JMP; LMP7; LMP7MGC1491; Low molecular mass protein 7; low molecular weight protein 7; Macropain subunit C13; Multicatalytic endopeptidase complex subunit C13; NKJO; protease component C13; proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctionalpeptidase 7); proteasome catalytic subunit 3i; Proteasome Component C13; proteasome subunit beta 5i; proteasome subunit beta type-8; Proteasome subunit beta-5i; Proteasome Subunit Y2; proteasome-related gene 7; PSMB5i; PSMB5iD6S216; PSMB8; Really interesting new gene 10 protein; RING10; RING10prote PSMB8 ALDD, D6S216, D6S216E, JMP, LMP7, NKJO, PRAAS1, PSMB5i, RING10 proteasome 20S subunit beta 8 proteasome subunit beta type-8|low molecular mass protein 7|low molecular weight protein 7|macropain subunit C13|multicatalytic endopeptidase complex subunit C13|protease component C13|proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)|proteasome catalytic subunit 3i|proteasome component C13|proteasome subunit Y2|proteasome subunit beta 5i|proteasome subunit beta 8|proteasome-related gene 7|really interesting new gene 10 protein

For more info on PSMB8, check out the PSMB8 Infographic

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In this infographic, you will see the following information for PSMB8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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