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- Table of Contents
CRB2 antibody (A05652) performs well in ELISA, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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WB Validation in Human Kidney
Loading: 15 μg of tissue lysate Antibodies: CRB2 A05652, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Loading: 15 μg of tissue lysate Antibodies: CRB2 A05652, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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Anti-Protein crumbs homolog 2 CRB2 Antibody
CRB2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, WB |
Recommend Dilution
Application
Dilution
Species
CRB2 antibody can be used for detection of CRB2 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human
mouse
and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Protein crumbs homolog 2 CRB2 Antibody
View all CRB2 Antibodies
View all CRB2 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
CRB2 antibody can be stored at 4°C, stable for up to one year.
Host
Rabbit
Contents
CRB2 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal CRB2 antibody was raised against a 17 amino acid peptide near the center of human CRB2. The immunogen is located within amino acids 460 - 510 of CRB2.
Cross-Reactivity
At least three isoforms of CRB2 are known to exist; this antibody will detect all three. CRB2 antibody is predicted to not cross-react with other members of the CRB family.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
134.3 kDa
Background of CRB2
CRB2 (Crumbs homolog 2), like its homologs CRB1 and CRB3, is similar to the Drosophila crumbs protein and is expressed in retina, brain and kidney. Along with other proteins, the Crumbs proteins form a complex that help set up cell polarity in developing neuroepithelial cells. At the onset of neural specification, embryonic stem cells (ESCs) upregulate CRB2, which then localizes apically in neural rosettes. Gain- and loss-of-function studies of CRB2 have shown that CRB2 is essential for the stabilization of other polarity proteins. Unlike CRB1, mutations in CRB2 do not appear to play a role in retinitis pigmentosa or in Leber congenital amaurosis.
Cite This Product
Anti-Protein crumbs homolog 2 CRB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05652)
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Product Promise
We promise Anti-Protein crumbs homolog 2 CRB2 Antibody performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A05652
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026