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Product Info Summary
| SKU: | M00513 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-Protein delta homolog 1 DLK1 Monoclonal Antibody
View all Pref-1/DLK1/FA1 Antibodies
SKU/Catalog Number
M00513
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Protein delta homolog 1 DLK1 Monoclonal Antibody catalog # M00513. Tested in ELISA, Flow Cytometry, IF, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein delta homolog 1 DLK1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00513)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
3A10
Isotype
IgG
Immunogen
Purified recombinant fragment of human DLK1 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00513 is reactive to DLK1 in Human
Applications
M00513 is guaranteed for ELISA, Flow Cytometry, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
41.3kDa
Background of Pref-1/DLK1/FA1
GATA4, also know as GATA-binding factor 4, acts in cooperation with NKX2-5 as a transcriptional activator of ANF. It binds to the consensus sequence 5'-AGATAG-3' on DNA. GATA4 is critical for the enlargement of cardiac myocytes. Mutations in GATA4 have been linked with congenital heart malfunctions and malformations. Anti-GATA4 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Figure 4. ELISA validation of DLK1 using Anti-Protein delta homolog 1 DLK1 Monoclonal Antibody (M00513).
ELISA analysis of DLK1 antibody.
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Figure 3. Flow Cytometry validation of DLK1 using Anti-Protein delta homolog 1 DLK1 Monoclonal Antibody (M00513).
Flow cytometric (FCM) analysis of NIH/3T3 cells using DLK1 Monoclonal Antibody (green) and negative control (red).
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Figure 2. Immunofluorescent staining data of DLK1 using Anti-Protein delta homolog 1 DLK1 Monoclonal Antibody (M00513).
Immunofluorescence (IF) analysis of U251 cells using DLK1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Figure 1. Western blotting validation for Anti-Protein delta homolog 1 DLK1 Monoclonal Antibody M00513
Western Blot (WB) analysis using DLK1 Monoclonal Antibody against human DLK1 (AA: 174-349) recombinant protein.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For DLK1 (Source: Uniprot.org, NCBI)
Gene Name
DLK1
Full Name
Protein delta homolog 1
Weight
41.3kDa
Alternative Names
delta-like 1 homolog (Drosophila); DLK; DLK1; DLK-1; FA1; FA1fetal antigen 1; pG2; pG2delta-like homolog (Drosophila); preadipocyte factor 1; Pref1; Pref-1; protein delta homolog 1; secredeltin; ZOG DLK1 DLK, DLK-1, Delta1, FA1, PREF1, Pref-1, ZOG, pG2 delta like non-canonical Notch ligand 1 protein delta homolog 1|delta-like 1 homolog|fetal antigen 1|preadipocyte factor 1|secredeltin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DLK1, check out the DLK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DLK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein delta homolog 1 DLK1 Monoclonal Antibody (M00513)
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No publications found for M00513
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