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Product Info Summary
| SKU: | A30534 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-PRP31 Antibody
SKU/Catalog Number
A30534
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PRP31 Antibody (Catalog# A30534). Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PRP31 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30534)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human PRP31. Immunogen sequence location: 331-380
Reactive Species
A30534 is reactive to PRPF31 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
55.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30534 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 2000-20000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western blot analysis of lysates from HeLa cells, using PRP31 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-PRP31 Antibody (A30534)
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Customer Q&As
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3 Customer Q&As for Anti-PRP31 Antibody
Question
My question regarding product A30534, anti-PRP31 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Carter
Verified customer
Asked: 2019-04-03
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30534 anti-PRP31 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-04-03
Question
We are currently using anti-PRP31 antibody A30534 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on feline tissues as well?
S. Kulkarni
Verified customer
Asked: 2014-03-19
Answer
The anti-PRP31 antibody (A30534) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-19
Question
Does anti-PRP31 antibody A30534 work on horse WB with mammary gland?
C. Miller
Verified customer
Asked: 2013-09-23
Answer
Our lab technicians have not validated anti-PRP31 antibody A30534 on horse. You can run a BLAST between horse and the immunogen sequence of anti-PRP31 antibody A30534 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse mammary gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-09-23
