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- Table of Contents
KLK3 antibody (A01505-3) performs well in ELISA, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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IF analysis of Prostate Specific Antigen/KLK3 using anti-Prostate Specific Antigen/KLK3 antibody (A01505-3).
Paraffin-embedded tumor sections were used to detect Prostate Specific Antigen (PSA/KLK3). PC-3 cells were inoculated into nude mouse to establish tumors. Tumor-bearing mice were subsequently treated with docetaxel, while control mouse remained untreated. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:500 rabbit anti-Prostate Specific Antigen/KLK3 Antibody (A01505-3) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (H+L) (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 45 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Paraffin-embedded tumor sections were used to detect Prostate Specific Antigen (PSA/KLK3). PC-3 cells were inoculated into nude mouse to establish tumors. Tumor-bearing mice were subsequently treated with docetaxel, while control mouse remained untreated. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:500 rabbit anti-Prostate Specific Antigen/KLK3 Antibody (A01505-3) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (H+L) (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 45 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Prostate Specific Antigen/KLK3 Antibody Picoband®
KLK3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IHC, WB |
Tested Applications
KLK3 antibody was positively detected by WB in:
human LNCAP whole cell, human LNCAP whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
KLK3 antibody was positively detected by IHC in:
human prostatic cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Prostate Specific Antigen/KLK3 Antibody Picoband®
View all KLK3 Antibodies
View all KLK3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Prostate Specific Antigen/KLK3 recombinant protein (Position: A64-D255).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
34 kDa
Calculated Molecular Weight
28.7 kDa
Background of KLK3
Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a glycoprotein enzyme encoded in humans by the KLK3 gene. KLK3 is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. This gene is mapped to 19q13.33. KLK3 is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. KLK3 is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely. It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm. It is not a unique indicator of prostate cancer, but may also detect prostatitis or benign prostatic hyperplasia.
Cite This Product
Anti-Prostate Specific Antigen/KLK3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01505-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
88%
Moderate Homology
Cow
59%
Low homology
Mouse
57%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for KLK3 antibody
Immunohistochemistry (IHC) protocol for KLK3 antibody
Publications
Application:
Species:
Mechanistic Insights Into the Anti-Benign Prostatic Hyperplasia Effect of Licorice Water Extract via p53-Mediated Apoptosis and Cell Cycle Arrest: A Network Pharmacology-Based Experimental Study
Published: 2026-04-10
Journal: MOLECULAR NUTRITION & FOOD RESEARCH
Application: WB
Impact Factor: 4.20
Species: Rat,Human
PMID: 41964254
Targeting benign prostate hyperplasia treatments: AR/TGF-β/NOX4 inhibition by apocynin suppresses inflammation and proliferation
Published: 2023-04-14
Journal: Journal of Advanced Research
Application: WB
Impact Factor: 10.70
Species: Human
PMID: 37061215
Purple Corn Extract Improves Benign Prostatic Hyperplasia by Regulating Prostate Cell Proliferation and Apoptosis
Published: 2022-04-25
Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Application: WB
Impact Factor: 5.90
Species: Human
PMID: 35466676
Review
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2 Reviews For Anti-Prostate Specific Antigen/KLK3 Antibody Picoband®
IHC using Anti-Ki67 antibody(A01505-3) showed clear nuclear staining with minimal background, revealing markedly reduced Ki67-positive tumor cells in docetaxel-treated PC-3 xenografts compared to control mouse.
| SKU | A01505-3 |
|---|---|
| Application | Immunohistochemistry |
| Sample | PC-3 xenograft mouse tissue |
| Sample Processing Description | (1) PC-3 cells were implanted in nude mice to form tumors as the control group; (2) tumor-bearing mice were treated with the drug docetaxel. |
| Other Reagents | Goat serum, DAPI, Anti-fade mounting medium |
| Primary Antibody | Prostate Specific Antigen/KLK3 Antibody Picoband® |
| Primary Incubation | 1:500, overnight at 4 ℃ |
| Secondary Antibody | Two-step IHC Detection Kit (Rabbit IgG) |
| Secondary Incubation | 1:500, 45 min at 37℃ |
| Detection | Imaging system:Leica DM2500 |
| Results Summary | Ki67 is a nuclear protein directly associated with cell proliferation and is widely recognized as a marker of proliferating cells due to its importance in evaluating tumor growth rate and patient prognosis. Higher Ki67 indices indicate more actively proliferating tumor cells, usually correlating with faster growth and increased aggressiveness. After treatment with docetaxel, extensive tumor cell death occurred, and proliferative activity was partially suppressed, resulting in fewer Ki67-positive tumor cells. These results clearly reflect the inhibitory effect of the drug on tumor proliferation. |
Dengxiao Zhang, Gansu Provincial People’s Hospital
Verified customer
Submitted 2026-01-30
Immunofluorescence using KLK3 antibody (A01505-3) showed clear and specific staining in PC-3 xenograft tumors, with markedly reduced PSA expression in tumors treated with docetaxel compared to untreated controls, consistent with expected drug effects.
| SKU | A01505-3 |
|---|---|
| Application | Immunofluorescence |
| Sample | PC-3 xenograft mouse tissue |
| Sample Processing Description | (1) PC-3 cells were implanted in nude mice to form tumors as the control group; (2) tumor-bearing mice were treated with the drug docetaxel. |
| Other Reagents | Goat serum, DAPI, Anti-fade mounting medium |
| Primary Antibody | Prostate Specific Antigen/KLK3 Antibody Picoband® |
| Primary Incubation | 1:500, overnight at 4 ℃ |
| Secondary Antibody | DyLight 594–conjugated Goat Anti-Rabbit IgG (H+L)) |
| Secondary Incubation | 1:500, 45 min at 37℃ |
| Detection | Imaging system:Leica DM2500 |
| Results Summary | PSA, or prostate-specific antigen, is a multifaceted tumor marker that can promote the proliferation and migration of prostate cancer cells, driving tumor growth. After treatment with docetaxel, its secretion is expected to decrease, which is confirmed by the experimental results showing markedly reduced PSA expression in the xenograft tumor cells following treatment. |
Dengxiao Zhang, Gansu Provincial People’s Hospital
Verified customer
Submitted 2026-01-26
Product Promise
We promise Anti-Prostate Specific Antigen/KLK3 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01505-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026