Anti-PSD95/DLG4 Antibody Picoband™

Product Name

Anti-PSD95/DLG4 Antibody Picoband™

View all PSD-95 Antibodies

SKU/Catalog Number

PB9802

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PSD95/DLG4 Antibody Picoband™ catalog # PB9802. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PSD95/DLG4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9802)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PSD95 recombinant protein (Position: H581-L724). Human PSD95 shares 100% amino acid (aa) sequence identity with both mouse and rat PSD95.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9802 is reactive to DLG4 in Human, Mouse, Rat

Applications

PB9802 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

100 kDa

Calculated molecular weight

80.495kDa

Background of PSD-95

DLG4 discs large homolog 4, also known as PSD95 or SAP-90, is a protein that in humans is encoded by the DLG4 gene. It is a member of the membrane-associated guanylate kinase (MAGUK) family. This gene is mapped to 17p13.1. DLG4 can heteromultimerize with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Overexpression of DLG4 in hippocampal neurons could drive maturation of glutamatergic synapses. DLG4 can orchestrate synaptic development and it has a role in synapse stabilization and plasticity. Ubiquitination of DLG4 through an MDM2-mediated pathway can regulate AMPA receptor surface expression during synaptic plasticity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PSD95 using anti-PSD95 antibody (PB9802).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Brain Tissue Lysate,
Lane 2: mouse brain Tissue Lysate,
Lane 3: human SHG-44 Whole Cell Lysate,
Lane 4: human U-87MG Whole Cell Lysate
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSD95 antigen affinity purified polyclonal antibody (Catalog # PB9802) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSD95 at approximately 100KD. The expected band size for PSD95 is at 80KD.

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Figure 2. Flow Cytometry analysis of A431 cells using anti-PSD95 antibody (PB9802).
Overlay histogram showing A431 cells stained with PB9802 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSD95 Antibody (PB9802,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For DLG4 (Source: Uniprot.org, NCBI)

Alternative Names

discs, large homolog 4 (Drosophila); disks large homolog 4; DLG4; FLJ97752; FLJ98574; Postsynaptic density protein 95; post-synaptic density protein 95; PSD95; PSD-95; PSD95SAP90SAP-90discs large homolog 4; SAP90; Synapse-associated protein 90; Tax interaction protein 15 DLG4 MRD62, PSD95, SAP-90, SAP90 discs large MAGUK scaffold protein 4 disks large homolog 4|Tax interaction protein 15|discs large homolog 4|post-synaptic density protein 95|synapse-associated protein 90

For more info on DLG4, check out the DLG4 Infographic

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In this infographic, you will see the following information for DLG4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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